文献类型： 外文期刊

作者： Qian, Zong-Yao ¹ ; Li, Zhi-Gang ¹ ; Ma, Jian ¹ ; Gong, Ting-Ting ¹ ; Xian, Qi-Ming ¹ ;

作者机构： 1.Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing 210023, Jiangsu, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Agr Qual Stand & Testing Technol Res, Xinjiang 830091, Peoples R China

关键词： Microcystin;MSPD;Vegetable;Determination;Lake Taihu;LC-MS/MS

期刊名称：TALANTA （ 影响因子：6.057； 五年影响因子：5.386 ）

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收录情况： SCI

摘要： A selective and sensitive method for the simultaneous determination of five common microcystins (MC-LR,-RR,-YR,-LW,-LF) in various vegetables was established using Matrix Solid-Phase Dispersion (MSPD) followed by high performance liquid chromatography-mass spectrometry detection. To optimize the pretreatment procedure for extracting the microcystins from three vegetable matrices (tomato, cucumber and spinacia, which represented different colors), Box-Behnken design was employed to optimize the main factors affecting the extraction efficiency, including sample/dispersant mass ratio, as well as the proportion mixture dispersant and the volumes of solvent. Based on the optimum conditions, the intra-day and inter-day variability for each microcystins in all vegetable samples were less than 8.6%, with the recoveries ranging from 71.9% to 96.5%. The limits of detection and quantitation of the method were 13.0 mu g/kg (dw) and 43.0 pg/kg (dw), respectively. The established method was successfully applied to the analysis of MCs in vegetable samples which were collected by Lake Taihu. The procedure promising to be relative small sample size and short pretreatment time for the assay that can be used for monitoring MCs concentrations in vegetables.