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Porcine parvovirus infection activates inflammatory cytokine production through Toll-like receptor 9 and NF-kappa B signaling pathways in porcine kidney cells

文献类型： 外文期刊

作者： Zhou, Yong 1 ; Jin, Xiao-hui 1 ; Jing, Ya-xing 2 ; Song, Yue 2 ; He, Xiao-xiang 1 ; Zheng, Lan-Lan 2 ; Wang, Ya-bin 2 ; W 1 ;

作者机构： 1.Henan Agr Univ, Coll Anim Sci & Vet Med, 63 Nongye Rd, Zhengzhou 450002, Henan, Peoples R China

2.Key Lab Animderived Food Safety Henan Prov, Zhengzhou 450002, Henan, Peoples R China

3.Henan Acad Agr Sci, Henan Prov Key Lab Anim Immunol, Minist Agr, Key Lab Anim Immunol, Zhengzhou 450002, Hena

关键词： Porcine parvovirus;IL-6;NF-kappa B signaling pathway;Toll-like receptor

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Porcine parvovirus virus (PPV) is an animal virus that has caused high economic losses for the swine industry worldwide. Previous studies demonstrated that PPV infection induced significant production of interleukin 6 (IL-6) in vitro and in vivo. However, the inflammatory cytokines and specific signaling pathways induced during PPV infection remain largely unknown. In the present study, we analyzed the expression levels of IL-6 in PPV-infected porcine kidney 15 (PK-15) and the results showed that PPV infection induced the increase of IL-6 mRNA expression in a dose-dependent manner. We also detected the expression of toll-like receptor 9 (TLR9) signaling proteins in the mRNA expressing level, when compared with the control group, the TLR9 expression in mRNA level increased at 24 h in PK-15 cells after PPV infection and reached the peak level at 48 h. In addition, the transcript profile of nuclear factor kappa B (NF-kappa B) signal pathway relating genes (MyD88, IRAK1, TRAF6, TAK1 alpha, I kappa B kappa B and NF-kappa B) expression levels increased at different times. Furthermore, to verify the IL-6 expression was specific with the TLR9 expression and then by activating the NF-kappa B signal pathway, TLR9 and NF-kappa B specific inhibitors were applied during PPV infection, separately, the result indicated that the expression of IL-6 was decreased after inhibitor treatment. Taken together, PPV infection significantly induced IL-6 expression and this induction depended on NF-kappa B activation and TLR9 signaling pathways in PK-15 cell.

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[1]Porcine parvovirus capsid protein expressed in Escherichia coli self assembles into virus-like particles with high immunogenicity in mice and guinea pigs. Ji, Pengchao,Zhou, Enmin,Ji, Pengchao,Liu, Yunchao,Chen, Yumei,Jiang, Dawei,Zhao, Baolei,Wang, Jvcai,Chai, Shujun,Zhang, Gaiping,Wang, Aiping,Zhang, Gaiping,Jiang, Dawei,Zhao, Baolei,Wang, Jvcai,Zhang, Gaiping,Zhang, Gaiping.

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