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High production, purification, biochemical characterization and gene analysis of a novel catalase from the thermophilic bacterium Ureibacillus thermosphaericus FZSF03

文献类型： 外文期刊

作者： Jia, Xianbo 1 ; Lin, Xinjian 2 ; Tian, Yandan 2 ; Chen, Jichen 2 ; You, Minsheng 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Inst Appl Ecol, Fuzhou, Fujian, Peoples R China

2.Fujian Acad Agr & Sci, Inst Soil & Fertilizer, Fuzhou, Fujian, Peoples R China

关键词： Ureibacillus thermosphaericus;Catalase;High production

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

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收录情况： SCI

摘要： A catalase-producing thermophilic bacterium, Ureibacillus thermosphaericus FZSF03, was isolated from high-temperature compost. Catalase production in this strain increased 31 times and reached 57,630 U/mL after optimization in a shake flask, which might represent the highest catalase activity level among reported wild strains. This catalase was further purified and identified. The purified enzyme showed a specific activity of 219,360 U/mg, higher than many other catalases. The molecular weight of this enzyme is 52 kDa according to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the enzyme was identified as a monofunctional haeme catalase of Ureibacillus thermosphaericus by liquid chromatography-mass spectrometry (LC-MS)/MS. The optimal reaction temperature for this catalase was found to be 60 degrees C. Stability was observed at 60 degrees C and at a pH of 10.0, indicating the superiority of this enzyme at a high temperature and under alkaline conditions. Therefore, this catalase is a prospective candidate for industrial production and applications. The gene encoding this catalase is 1503 bp. As the amino acid sequence shows low similarity with other catalases, we suggest that this is a novel monofunctional haeme catalase. (C) 2017 Elsevier B.V. All rights reserved.

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