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Porcine glucagon-like peptide-2 microspheres ameliorate inflammation in lipopolysaccharide-challenged weaning piglets

文献类型： 外文期刊

作者： Wu, J. 1 ; Qi, K. K. 1 ; Xu, Z. W. 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Sci, Hangzhou 310021, Zhejiang, Peoples R China

关键词： anti-inflammation;lipopolysaccharide;microspheres;porcine glucagon-like peptide-2;trophic effects

期刊名称：JOURNAL OF ANIMAL SCIENCE （ 影响因子：3.159； 五年影响因子：2.912 ）

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收录情况： SCI

摘要： This study aimed to investigate the effects of porcine [gly2] glucagon-like peptide-2 (p[gly2] GLP-2) microspheres on lipopolysaccharide-challenged piglets and to evaluate efficacy of microspheres for administration compared with more conventional administration. Eighteen 21-d-old Duroc female piglets were randomly assigned into 3 groups: the control group (intraperitoneal injection with 3 mL saline solution daily), the glucagon-like peptide-2 (GLP-2) group (intraperitoneal injection with 3 mL p[gly2] GLP-2 at 20 nmol/kg BW daily), and the microsphere (MS) group (intraperitoneal injection with 100 mg p[gly2] GLP-2 microsphere suspension at Day 1). On Day 8, all piglets were injected with 100 mu g lipopolysaccharide/kg BW. Results showed that administration of p[gly2] GLP-2 microspheres decreased the D-lactic acid and methane dicarboxylic aldehyde content of the serum and increased the villus height and villus crypt ratio in the duodenum and ileum. Inducible nitric oxide synthase activity in the duodenum and ileum decreased, whereas enzyme activity for sucrose and Na-K adenosine triphosphatase in the ileum increased with treatment of p[gly2] GLP-2 microspheres. In the MS group, we observed downregulation of IL-8, TNF-alpha, IFN-gamma, and GLP-2R mRNA expression in the ileum, upregulation of KI-67 positive cell expression in the duodenum and Bcl-2 positive cell expression in the ileum, and downregulation of GLP-2 receptor positive cell expression in the ileum. One injection of p[gly2] GLP-2 microspheres was as effective as p[gly2] GLP2 administered for 7 d. Results suggested that p[gly2] GLP-2 can be a candidate agent for ameliorating weaning stress in piglets and that the use of microspheres is an ideal delivery system for GLP-2.

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