Effect of porcine glucagon-like peptides-2 on tight junction in GLP-2R+IPEC-J2 cell through the PI(3)k/Akt/mTOR/p70(S6K) signalling pathway
文献类型： 外文期刊
作者： Qi, K. K. 1 ; Sun, Y. Q. 1 ; Wan, J. 1 ; Deng, B. 1 ; Men, X. M. 1 ; Wu, J. 1 ; Xu, Z. W. 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Sci, 198 Shiqiao Rd, Hangzhou 310021, Zhejiang, Peoples R China
关键词： porcine glucagon-like peptide-2;IPEC-J2;tight junction protein;PI(3)k;Akt;mTOR;p70(S6K)
期刊名称：JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION （ 影响因子：2.13； 五年影响因子：2.322 ）
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收录情况： SCI
摘要： Because of rare glucagon-like peptide-2 (GLP-2) receptor (+) cells within the gut mucosa, the molecular mechanisms transducing the diverse actions of GLP-2 remain largely obscure. This research identified the naturally occurring intestinal cell lines that endogenously express GLP-2R and determined the molecular mechanisms of the protective effects of GLP-2-mediated tight junctions (TJ) in GLP-2R (+) cell line. (i) Immunohistochemistry results showed that GLP-2R is localised to the epithelia, laminae propriae and muscle layers of the small and large bowels of newborn piglets. (ii) GLP-2R expression was apparent in the cytoplasm of endocrine cells in IPEC-J2 cell lines. (iii) The protein expressions of ZO-1, claudin-1, occludin, p-PI3K, p-Akt, p-mTOR and p-p70(S6K) significantly (p<0.05) increased in GLP-2-treated IPEC-J2 cells, and all of them significantly (p<0.05) decreased when LY-294002 or rapamycin was added. GLP-2 improves intestinal TJ expression of GLP-2R (+) cells through the PI(3)k/Akt/mTOR/p70(S6K) signalling pathway.
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