您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Overexpression of a PIP1 Gene from Salicornia bigelovii in Tobacco Plants Improves Their Drought Tolerance

文献类型： 外文期刊

作者： Sun, Xiaobo 1 ; Deng, Yanming 1 ; Liang, Lijian 1 ; Jia, Xinping 1 ; Xiao, Zheng 1 ; Su, Jiale 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Leisure Agr, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Jiangsu, Peoples R China

关键词： aquaporin;quantitative real-time PCR;subcellular localization;transgenic tobacco

期刊名称：JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE （ 影响因子：1.144； 五年影响因子：1.617 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Aquaporin (AQP) proteins can transport water and other small molecules through cellular membranes and are one of the first targets of stress-induced signaling in plants. A number of AQP genes have been identified from glycophytes, and their functions have been studied. However, the reports on AQPs from halophytes and their precise role in abiotic stress response are still rare. In this study, we have identified a PIP1 subgroup AQP gene, designated SbPIP1, from the euhalophyte Salicornia bigelovii and characterized it by overexpressing in tobacco plants. SbPIP1 transcript was induced by cold, but suppressed by NaCl and polyethylene glycol (PEG). Transient expression of GFP (green fluorescent protein)-SbPIP fusion protein indicated its localization in the plasma membrane. Overexpression of SbPIP1 in tobacco (Nicotiana tabacum) plants increased their drought tolerance. Leaf protoplasts from transgenic tobacco plants absorbed water more quickly than those from wild type (WT) plants when they were put into hypotonic solution. In addition, the transgenic tobacco plants possessed higher relative water content (RWC) and proline content, but lower levels of malondialdehyde (MDA) and less ion leakage (IL) when compared with WT under the treatment of the different concentrations of PEG. Taken together, our results demonstrate that heterologous expression of SbPIP1 in tobacco plants confers them drought stress tolerance by reducing membrane injury and increasing the ability to retain water.

  • 相关文献

[1]Molecular cloning and characterization of two novel DREB genes encoding dehydration-responsive element binding proteins in halophyte Suaeda salsa. Sun, Xiao-Bo,Ma, Hong-Xiang,Jia, Xin-Ping,Ye, Xiao-Qing,Chen, Yu.

[2]Heterologous expression of GmSIP1;3 from soybean in tobacco showed and growth retardation and tolerance to hydrogen peroxide. Zhang, Dayong,Huang, Yihong,Wan, Qun,Xu, Zhaolong,Shao, Hongbo,Shao, Hongbo,Kumar, Manoj,Pandey, Girdhar K..

[3]A P4-ATPase Gene GbPATP of Cotton Confers Chilling Tolerance in Plants. Liu, Tingli,Guo, Shiwei,Lian, Ziyi,Chen, Fei,Yang, Yuwen,Chen, Tianzi,Ling, Xitie,Liu, Aiming,Zhang, Baolong,Wang, Rongfu.

[4]Identification and differential expression of two isogenes encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase in Glycine max. Zhang, Man,Li, Kai,Liu, Jianyu,Yu, Deyue,Zhang, Man. 2012

[5]Genome-wide identification of the auxin response factor (ARF) gene family and expression analysis of its role associated with pistil development in Japanese apricot (Prunus mume Sieb. et Zucc). Gao, Zhihong,Huo, Ximei,Xu, Yanshuai,Shi, Ting,Ni, Zhaojun,Song, Juan,Sun, Hailong.

[6]Selection of appropriate reference genes in eggplant for quantitative gene expression studies under different experimental conditions. Zhou, Xiaohui,Liu, Jun,Zhuang, Yong.

[7]De novo characterization of the Iris lactea var. chinensis transcriptome and an analysis of genes under cadmium or lead exposure. Gu, Chun-Sun,Liu, Liang-Qin,Zhang, Yong-Xia,Wang, Zhi-Quan,Yuan, Hai-Yan,Huang, Su-Zhen,Gu, Chun-Sun,Liu, Liang-Qin,Zhang, Yong-Xia,Wang, Zhi-Quan,Yuan, Hai-Yan,Huang, Su-Zhen,Deng, Yan-Ming. 2017

[8]Genome-wide identification and expression analysis of beta-galactosidase family members during fruit softening of peach [Prunus persica (L.) Batsch]. Guo, Shaolei,Song, Juan,Zhang, Binbin,Jiang, Hang,Ma, Ruijuan,Yu, Mingliang,Guo, Shaolei,Jiang, Hang,Guo, Shaolei,Song, Juan,Zhang, Binbin,Jiang, Hang,Ma, Ruijuan,Yu, Mingliang. 2018

[9]Genome-wide characterization of the ankyrin repeats gene family under salt stress in soybean. Zhang, Dayong,Wan, Qun,He, Xiaolan,Ning, Lihua,Huang, Yihong,Xu, Zhaolong,Liu, Jia,Shao, Hongbo,Shao, Hongbo.

[10]Cloning and expression analysis of PpSUT2 encoding a sucrose transporter in pear. Tang, J.,Lin, J.,Zhang, B. L.,Wang, Z. H.,Li, X. G.,Chang, Y. H.,Tang, J.. 2014

[11]Genome-wide analysis of the TPX2 family proteins in Eucalyptus grandis. Gan, Siming,Du, Pingzhou,Yao, Yuan,Wang, Yuqi,Du, Pingzhou,Yao, Yuan,Xie, Qiaoli,Wu, Ai-Min,Du, Pingzhou,Yao, Yuan,Xie, Qiaoli,Wu, Ai-Min,Du, Pingzhou,Wang, Jinyan,Zhang, Baolong,Kumar, Manoj,Wang, Yuqi. 2016

[12]Investigation on subcellular localization of Rice stripe virus in its vector small brown planthopper by electron microscopy. Deng, Jinhua,Li, Shuo,Ji, Yinghua,Zhou, Yijun,Deng, Jinhua,Hong, Jian. 2013

[13]Characterization and subcellular localization of an RNA silencing suppressor encoded by Rice stripe tenuivirus. Xiong, Ruyi,Wu, Jianxiang,Zhou, Xueping,Zhou, Yijun. 2009

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台