文献类型： 外文期刊

作者： Wu, M. ¹ ; Li, B. ¹ ; Liu, P. ¹ ; Weng, Q. ¹ ; Zhan, J. ³ ; Chen, Q. ¹ ;

作者机构： 1.Fujian Acad Agr Sci, Inst Plant Protect, Fujian Key Lab Monitoring & Integrated Management, Fuzhou 350003, Fujian, Peoples R China

2.Xiamen Univ, Sch Life Sci, Xiamen Key Lab Plant Genet, Xiamen 361102, Peoples R China

3.Fujian Agr & Forestry Univ, Fujian Taiwan Joint Ctr Ecol Control Crop Pests, Fuzhou 350002, Fujian, Peoples R China

关键词： genetic diversity;microsatellite marker;Phytophthora sojae;virulence analysis

期刊名称：PLANT PATHOLOGY （ 影响因子：2.59； 五年影响因子：2.924 ）

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收录情况： SCI

摘要： Phytophthora sojae is a destructive soilborne pathogen causing seedling damping-off and root rot of soybean (Glycine max). The goal of this study was to determine the genetic structure of P. sojae populations in Fujian, China. Nine microsatellite markers were used to investigate the genetic variation in 19 P. sojae populations, sampled from Fujian Province and northeastern China (Jilin and Heilongjiang Provinces) between 2002 and 2013. Overall, a low genetic diversity, Hardy-Weinberg disequilibrium, and an (r) over bar (d) index (an index of association) that was significantly different from zero were detected in populations; these results were consistent with self-fertilization and clonal modes of reproduction for this pathogen. However, using Bayesian Markov chain Monte Carlo approach, principal component analysis and neighbour joining (NJ) algorithm, the Fujian P. sojae populations clustered into three distinct groups, one of which included most isolates of the northeast populations. What is more, significant estimates of pairwise fixation indices (F-ST) were detected between most populations, especially in different clusters. It is hypothesized that the cropping system used, the limited dispersal ability, and human-mediated gene flow may account for the observed genetic structure of P. sojae populations in Fujian, China. In addition, a high virulence frequency of the pathogen on different cultivars carrying known major R genes for resistance, and a rapid increase in virulence frequency, indicated that these major R genes should not be used to manage seedling damping-off and root rot diseases of soybean (Glycine max).