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The recombinant pseudorabies virus expressing African swine fever virus CD2v protein is safe and effective in mice

文献类型： 外文期刊

作者： Feng, Zhihua 1 ; Chen, Jianghua 1 ; Liang, Wangwang 1 ; Chen, Wenzhi 1 ; Li, Zhaolong 1 ; Chen, Q. 1 ; Cai, Shaoli 1 ;

作者机构： 1.Fujian Normal Univ, Biomed Res Ctr South China, Fujian Key Lab Innate Immune Biol, Qishan Campus, Fuzhou 350117, Fujian, Peoples R China

2.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350117, Fujian, Peoples R China

关键词： African swine fever virus; CD2v; Pseudorabies virus; vaccine; immunity

期刊名称：VIROLOGY JOURNAL （ 影响因子：4.099； 五年影响因子：3.719 ）

ISSN：

年卷期： 2020 年 17 卷 1 期

页码：

收录情况： SCI

摘要： Background African swine fever (ASF) leads to high mortality in domestic pigs and wild boar and is caused by the African swine fever virus (ASFV). Currently, no vaccine is commercially available for prevention, and the epidemic is still spreading. Here, we constructed a recombinant pseudorabies virus (PRV) (PRV-Delta gE/Delta gI/Delta TK-(CD2v)) that expresses the CD2v protein of ASFV and evaluated its effectiveness and safety as a vaccine candidate in mice. Methods A homologous recombination fragment containing ASFV CD2v was synthesized and co-transfected into HEK 293 T cells, a knockout vector targeting the PRV TK gene. The transfected cells were infected with PRV-Delta gE/Delta gI, and the recombinant strain (PRV-Delta gE/Delta gI/Delta TK-(CD2v)) was obtained by plaque purification in Vero cells. The expression of ASFV CD2v in the recombinant virus was confirmed by sequencing, Western blotting, and immunofluorescence analysis, and the genetic stability was tested in Vero cells over 20 passages. The virulence, immunogenicity and protective ability of the recombinant virus were further tested in a mouse model. Results The PRV-Delta gE/Delta gI/Delta TK-(CD2v) recombinant strain is stable in Vero cells, and the processing of CD2v does not depend on ASFV infection. The vaccination of PRV-Delta gE/Delta gI/Delta TK-(CD2v) causes neither pruritus, not a systemic infection and inflammation (with the high expression of interleukin-6 (IL6)). Besides, the virus vaccination can produce anti-CD2v specific antibody and activate a specific cellular immune response, and 100% protect mice from the challenge of the virulent strain (PRV-Fa). The detoxification occurs much earlier upon the recombinant virus vaccination and the amount of detoxification is much lower as well. Conclusions The PRV-Delta gE/Delta gI/Delta TK-(CD2v) recombinant strain has strong immunogenicity, is safe and effective, and maybe a potential vaccine candidate for the prevention of ASF and Pseudorabies.

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