您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Butyric acid regulates progesterone and estradiol secretion via cAMP signaling pathway in porcine granulosa cells

文献类型： 外文期刊

作者： Lu, Naisheng 1 ; Li, Mengjiao 1 ; Lei, Hulong 1 ; Jiang, Xueyuan 1 ; Tu, Weilong 1 ; Lu, Yang 1 ; Xia, Dong 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Shanghai Engn Res Ctr Breeding Pig, Inst Anim Husb & Vet Sci, Shanghai 201106, Peoples R China

关键词： Butyric acid;Porcine granulosa cells;Progesterone;Estradiol;cAMP signaling pathway

期刊名称：JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：4.292； 五年影响因子：4.216 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Butyric acid (BA), one of the short chain fatty acids (SCFAs), has positive actions on the metabolism, inflammation, etc. However, whether it influences the reproductive physiology and if so the detail mechanism involved has not yet been determined. In this study, the porcine granulosa cells (PGCs) were treated with gradient concentrations of BA. After 24 h culture, 0.05 mM BA significantly stimulated the progesterone (P-4) secretion (P < 0.05), 5 mM and 10 mM BA significantly inhibited the P-4 secretion (P < 0.05). Simultaneously, BA up-regulated the estradiol (E-2) secretion in a dose dependent manner, 5 mM and 10 mM BA significantly promoted the E-2 level (P < 0.05). In addition, 10 mM BA significantly promoted the G-protein-coupled receptor 41/43 mRNA (P < 0.05). Interestingly, 5 mM BA treatment significantly down-regulated cyclic adenosine monophosphate (cAMP) content (P < 0.05), steroidogenic acute regulatory (StAR), steroidogenic factor 1 (SF1), P450scc in the mRNA and/or protein level (P < 0.05), and these actions were reversed by cAMP activator forskolin (FK). Moreover, the co-treatment of 5 mM BA and bupivacaine (BPC, the cAMP inhibitor) significantly accumulated the inhibition action of BPC on cAMP, the secretion of P-4, and the abundance of StAR mRNA (P < 0.05), inhibited the up-regulation of 5 mM BA on the E-2 secretion (P < 0.05). Further, the Global Proteome and KEGG pathway analysis found that 5 mM BA significantly up-regulated the I3LM80 proteins (P < 0.05), which is involved in the steroid biosynthesis signaling pathway. 5 mM BA significantly decreased the F2Z5G3 protein level (P < 0.05), and the cAMP signaling pathway. In conclusion, present findings for the first time demonstrated that BA could regulate the P-4 and E-2 hormone synthesis in PGCs via the cAMP signaling pathway.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台