Expression and Enzyme Activity of Catalase in Chilo suppressalis (Lepidoptera: Crambidae) Is Responsive to Environmental Stresses
文献类型: 外文期刊
作者: Lu, Yanhui 1 ; Bai, Qi 1 ; Zheng, Xusong 1 ; Lu, Zhongxian 1 ;
作者机构: 1.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China
2.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Peoples R China
关键词: catalase;antioxidant enzyme;peroxisome;environmental stress;Chilo suppressalis
期刊名称:JOURNAL OF ECONOMIC ENTOMOLOGY ( 影响因子:2.381; 五年影响因子:2.568 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Catalase (CAT) is an important antioxidant enzyme that protects organisms against oxidative stresses by eliminating hydrogen peroxide. In this study, we cloned and characterized a full-length cDNA of CAT from Chilo suppressalis (CsCAT) and examined the influence of environmental stresses on CsCAT expression and enzyme activity. The cDNA contains a 1659-bp open reading frame encoding a polypeptide of 553 amino acids most closely related (90.14%) to Papilio polytes catalases. The CsCAT was expressed in all developmental stages with the highest expression in the fat body, and the CsCAT enzyme activity closely mirrored its observed mRNA expression patterns. The CsCAT mRNA was up-regulated when the larvae were exposed to high temperature (>= 30 degrees C), insecticides (abamectin and chlorantraniliprole), chemicals (H2O2, CHP, CdCl2, and CuSO4 ), and a dead-end trap plant (vetiver grass), and the CsCAT enzyme activity again mirrored the observed CsCAT expression patterns. These results suggest that up-regulation of CsCAT may enhance the defense response of C. suppressalis by weakening the effects of environmental stresses, and provide insight into the role of CsCAT during development of C. suppressalis.
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