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Non-O1 Vibrio cholerae pathogen from Cyprinus carpio and control with anti-non-O1 V. cholerae egg yolk powder (IgY)

文献类型: 外文期刊

作者: Gao, Xiaojian 1 ; Chen, Nan 1 ; Zhang, Yue 1 ; Zhang, Xiaojun 1 ; Bing, Xuwen 2 ;

作者机构: 1.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou, Jiangsu, Peoples R China

2.Chinese Acad Fishery Sci, Minist Agr, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Utilizat, Wuxi, Peoples R China

关键词: Cyprinus carpio;Non-O1 Vibrio cholerae;Virulence gene;Egg yolk powder (IgY)

期刊名称:AQUACULTURE ( 影响因子:4.242; 五年影响因子:4.723 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Mass mortality in cultured common carp from a farm of Ganyu county, Jiangsu province, P. R. China was reported. The two dominated isolates named L1 and L2 from diseased common carps were confirmed as non-O1 Vibrio cholerae by biochemical characteristics and molecular tools. Virulence genes (ompW, hlyA, acfA, toxR, ace and rtxC genes) were found to be present in the isolates by PCR, which further confirmed pathogenicity of the isolates. The LD50 value of the isolate L1 to the common carp was 1.1 x 10(6) CFU/mL. In addition, the specific egg yolk powder (IgY) conferred significant protection of common carp against non-O1 V. cholerae. The specific IgY also exhibited significant bacteriolytic effects on the non-O1 V. cholerae pathogen. The results showed that the specific IgY should be an effective implemented feed additive for the biocontrol of non-O1 V. cholerae infection in aquaculture.

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