您好,欢迎访问上海市农业科学院 机构知识库!

Nitrous oxide production and mRNA expression analysis of nitrifying and denitrifying bacterial genes under floodwater disappearance and fertilizer application

文献类型: 外文期刊

作者: Riya, Shohei 1 ; Takeuchi, Yuki 1 ; Zhou, Sheng 2 ; Terada, Akihiko 1 ; Hosomi, Masaaki 1 ;

作者机构: 1.Tokyo Univ Agr & Technol, Dept Chem Engn, Fac Engn, 2-24-16 Naka Cho, Koganei, Tokyo 1848588, Japan

2.Shanghai Acad Agr Sci, Ecoenvironm Protect Res Inst, 1000 Jinqi Rd, Shanghai 201403, Peoples R China

关键词: Chemical fertilizer;Nitrous oxide;Soil surface;Floodwater disappearance;mRNA;nirK

期刊名称:ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH ( 影响因子:4.223; 五年影响因子:4.306 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: A pulse of nitrous oxide (N2O) emission has been observed following the disappearance of floodwater by drainage. However, its mechanism is not well understood. We conducted a column study to clarify the mechanism for N2O production during floodwater disappearance by using a microsensor and determining the bacterial gene expression. An increase in N2O flux was observed following floodwater disappearance after the addition of NH4+, with a corresponding increase in the concentrations of NO3- and dissolved N2O in the oxic and anoxic soil layers, respectively. The transcription level of the bacterial amoA mRNA did not change, while that of nirK mRNA increased sharply after an hour of floodwater disappearance. An additional anoxic soil slurry experiment demonstrated that the addition of NO3- induced the expression of nirK gene and caused a concomitant increase in N2O production. These findings suggest that NO3- production in the oxic layers is important as it provides a substrate and induces the synthesis of denitrification enzymes in the anoxic layer during N2O production.

  • 相关文献

[1]Assessing environmental impacts of organic and inorganic fertilizer on daily and seasonal Greenhouse Gases effluxes in rice field. Yuan, Jing,Sha, Zhi-min,Hassani, Danial,Cao, Lin-kui,Zhao, Zheng.

[2]A methodological approach to assess the combined reduction of chemical pesticides and chemical fertilizers for low-carbon agriculture. Wan, Nianfeng,Ji, Xiangyun,Jiang, Jiexian,Qiao, Hongxia,Huang, Kaihua.

[3]Influence of nitrogen loading and plant nitrogen assimilation on nitrogen leaching and N2O emission in forage rice paddy fields fertilized with liquid cattle waste. Riya, Shohei,Terada, Akihiko,Hosomi, Masaaki,Zhou, Sheng,Kobara, Yuso,Sagehashi, Masaki.

[4]Assessing impacts of alternative fertilizer management practices on both nitrogen loading and greenhouse gas emissions in rice cultivation. Zhao, Zheng,Yue, Yubo,Sha, Zhimin,Cao, Linkui,Li, Changsheng,Deng, Jia,Zhang, Hanlin,Gao, Maofang. 2015

作者其他论文 更多>>