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Pseudo-nitzschia simulans sp nov (Bacillariophyceae), the first domoic acid producer from Chinese waters

文献类型: 外文期刊

作者: Li, Yang 1 ; Huang, Chun Xiu 1 ; Xu, Guo Shuang 1 ; Lundholm, Nina 3 ; Teng, Sing Tung; Wu, Haiyan; Tan, Zhijun;

作者机构: 1.South China Normal Univ, Guangzhou Key Lab Subtrop Biodivers & Biomonitori, Coll Life Sci, 55 Zhongshan West Ave, Guangzhou 510631, Guangdong, Peoples R China

2.South China Normal Univ, Guangdong Prov Key Lab Hlth & Safe Aquaculture, Coll Life Sci, 55 Zhongshan West Ave, Guangzhou 510631, Guangdong, Peoples R China

3.Univ Copenhagen, Nat Hist Museum Denmark, Solvgade 83S, Copenhagen, Denma

关键词: Domoic acid;China;Pseudo-nitzschia;P. simulans

期刊名称:HARMFUL ALGAE ( 影响因子:4.273; 五年影响因子:5.824 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The genus Pseudo-nitzschia has attracted attention because of production of the toxin, domoic acid (DA), causing Amnesic Shellfish Poisoning (ASP). Pseudo-nitzschia blooms occur frequently in Chinese coastal waters, and DA has been detected in several marine organisms, but so far no Pseudo-nitzschia strains from Chinese waters have been shown to produce DA. In this study, monoclonal Pseudo-nitzschia strains were established from Chinese coastal waters and examined using light microscopy, electron microscopy and molecular markers. Five strains, sharing distinct morphological and molecular features differentiating them from other Pseudo-nitzschia species, represent a new species, Pseudo-nitzschia simulans sp. nov. Morphologically, the taxon belongs to the P. pseudodelicatissima group, cells possessing a central nodule and each stria comprising one row of poroids. The new species is characterized by the poroid structure, which typically comprises two sectors, each sector located near opposite margins of the poroid. The production of DA was examined by liquid chromatography tandem mass spectrometry (LC-MS/MS) analyses of cells in stationary growth phase. Domoic acid was detected in one of the five strains, with concentrations around 1.05-1.54 fg cell(-1). This is the first toxigenic diatom species reported from Chinese waters. (C) 2017 Elsevier B.V. All rights reserved.

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