The complete nucleotide sequence and genome organization of a novel betaflexivirus infecting Citrullus lanatus
文献类型： 外文期刊
作者：
Xin, Min
1
;
Zhang, Peipei
1
;
Liu, Wenwen
1
;
Ren, Yingdang
2
;
Cao, Mengji
3
;
Wang, Xifeng
1
;
作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
2.Henan Acad Agr Sci, Inst Plant Protect, Zhengzhou 450002, Henan, Peoples R China
3.Southwest Univ, Citrus Res Inst, Natl Citrus Engn Res Ctr, Chongqing 400712, Peoples R China
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： The complete nucleotide sequence of a novel positive single-stranded (+ss) RNA virus, tentatively named watermelon virus A (WVA), was determined using a combination of three methods: RNA sequencing, small RNA sequencing, and Sanger sequencing. The full genome of WVA is comprised of 8,372 nucleotides (nt), excluding the poly (A) tail, and contains four open reading frames (ORFs). The largest ORF, ORF1 encodes a putative replication-associated polyprotein (RP) with three conserved domains. ORF2 and ORF4 encode a movement protein (MP) and coat protein (CP), respectively. The putative product encoded by ORF3, of an estimated molecular mass of 25 kDa, has no significant similarity with other proteins. Identity and phylogenetic analysis indicate that WVA is a new virus, closely related to members of the family Betaflexiviridae. However, the final taxonomic allocation of WVA within the family is yet to be determined.
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