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Karyotypes and Distribution of Tandem Repeat Sequences in Brassica nigra Determined by Fluorescence in situ Hybridization

文献类型: 外文期刊

作者: Wang, Gui-xiang 1 ; He, Qun-yan; Macas, Jiri; Novak, Petr; Neumann, Pavel; Meng, De-xuan; Zhao, Hong 1 ; G 1 ;

作者机构: 1.Beijing Acad Agr & Forestry Sci, Key Lab Biol & Genet Improvement Hort Crops North, Beijing Key Lab Vegetable Germplasm Improvement, Beijing Vegetable Res Ctr,Minist Agr, Beijing 100097, Peoples R China

2.Beij

关键词: Brassica nigra;Fluorescence in situ hybridization;Karyotype;(Peri) centromere;Repeat sequence

期刊名称:CYTOGENETIC AND GENOME RESEARCH ( 影响因子:1.636; 五年影响因子:1.943 )

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收录情况: SCI

摘要: Whole-genome shotgun reads were analyzed to determine the repeat sequence composition in the genome of black mustard, Brassica nigra (L.) Koch. The analysis showed that satellite DNA sequences are very abundant in the black mustard genome. The distribution pattern of 7 new tandem repeats (BnSAT13, BnSAT28, BnSAT68, BnSAT76, BnSAT114, BnSAT180, and BnSAT200) on black mustard chromosomes was visualized using fluorescence in situ hybridization (FISH). The FISH signals of BnSAT13 and BnSAT76 provided useful cytogenetic markers; their position and fluorescence intensity allowed for unambiguous identification of all 8 somatic metaphase chromosomes. A karyotype showing the location and fluorescence intensity of these tandem repeat sequences together with the position of rDNAs and centromeric retrotransposons of Brassica (CRB) was constructed. The establishment of the FISH-based karyotype in B. nigra provides valuable information that can be used in detailed analyses of B. nigra accessions and derived allopolyploid Brassica species containing the B genome. (C) 2017 S. Karger AG, Basel

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