Identification of male-specific AFLP and SCAR markers in the dioecious plant Humulus scandens
文献类型: 外文期刊
作者: Li, Shu-Fen 1 ; Wang, Lian-Jun 2 ; Deng, Chuan-Liang 1 ; Gao, Wu-Jun 1 ;
作者机构: 1.Henan Normal Univ, Coll Life Sci, Jianshe Rd, Xinxiang 453007, Henan, Peoples R China
2.Hubei Acad Agr Sci, Inst Food Corps, Wuhan 430064, Hubei, Peoples R China
关键词: AFLP;Humulus scandens;Retrotransposable elements;SCAR;Sex-linked marker
期刊名称:MOLECULAR AND CELLULAR PROBES ( 2020影响因子:2.365; 五年影响因子:2.386 )
收录情况: SCI
摘要: In this study, 17 male-specific amplified fragment length polymorphism (AFLP) markers were identified between male and female Humulus scandens plants. BLAST analysis revealed that 7 of the 17 sex-linked sequences were highly similar to retrotransposons. Two stable male-specific sequence-characterized amplified regions (SCAR) markers were developed. These AFLP and SCAR markers are novel molecular probes that can be used efficiently to identify the genetic gender of H. scandens and may provide a basis for further investigations on the evolution of sex chromosomes. (C) 2017 Elsevier Ltd. All rights reserved.
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