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Molecular cloning and expression analysis of duplicated polyphenol oxidase genes reveal their functional differentiations in sorghum

文献类型： 外文期刊

作者： Yan, Song 1 ; Li, Sujuan 1 ; Zhai, Guowei 1 ; Lu, Ping 3 ; Deng, Hui; Zhu, Shan 2 ; Huang, Renliang 2 ; Shao, Jianfeng 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, 198 Shiqiao Rd, Hangzhou 310021, Zhejiang, Peoples R China

2.Jiangxi Acad Agr Sci, Rice Res Inst, Rice Natl Engn Lab, Nanchang 330200, Jiangxi, Peoples R China

3.Chinese Acad Agr

关键词： Sorghum;Polyphenol oxidase gene;Phenol color reaction;Transgene;Expression analysis;Abiotic stress

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

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收录情况： SCI

摘要： Polyphenol oxidase (PPO) is believed to play a role in plant growth, reproduction, and resistance to pathogens and pests. PPO causes browning of grains in cereals. In this study, genetic mapping of sorghum grain for phenol color reaction (PHR) was performed using a recombinant inbred line population. Only one locus was detected between SSR markers SM06072 and Xtxp176 on chromosome 6. Two linked orthologous genes (Sb06PPO1 and Sb06PPO2) within the mapped region were discovered and cloned. Transformation experiments using Nipponbare (a PHR negative rice cultivar) showed that Sb06PPO1 from LTR108 and two Sb06PPO2 alleles from both varieties could complement Nipponbare, whereas Sb06PPO1 from 654 could not. Subsequent quantitative real-time PCR (qPCR) experiments showed that Sb06PPO1 and Sb06PPO2 functioned diversely, Sb06PPO1 was mainly expressed in young panicles before flowering. Sb06PPO2 was strongly expressed in flowering panicles, especially in hulls and branches at filling stage. Moreover, the expression of Sb06PPO1 was found to be significantly up-regulated by exogenous ABA and salt, whereas Sb06PPO2 was not changed significantly, further demonstrating functional differentiation between the two genes.

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