文献类型： 外文期刊

作者： Zhu, Keyu ¹ ; Li, Miaomiao ¹ ; Dong, Lingli ¹ ; Zhang, Huaizhi ¹ ; Zhang, Deyun ² ; Lu, Ping ¹ ; Wu, Qiuhong ³ ; Xie, Jingzhong ¹ ; Chen, Yongxing ³ ; Guo, Guanghao ¹ ; Zhang, Panpan ⁴ ; Li, Beibei ³ ; Li, Wenling ³ ; Dong, Lei ³ ; Hou, Yikun ¹ ; Yang, Yijun ¹ ; Qiu, Dan ¹ ; Wang, Gaojie ¹ ; Huang, Baoge ¹ ; Cui, Xuejia ¹ ; Fu, Hongkui ¹ ; Yuan, Chengguo ⁶ ; Fahima, Tzion ⁷ ; Nevo, Eviatar ⁷ ; Li, Hongjie ³ ; Rong, Junkang ⁸ ; Hua, Wei ³ ; Liu, Zhiyong ¹ ;

作者机构： 1.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Seed Innovat, Beijing, Peoples R China

2.Chaozhou Hybribio Biochem Ltd, Chaozhou, Peoples R China

3.Inst Biotechnol, Xianghu Lab, Hangzhou, Peoples R China

4.Yunnan Acad Agr Sci, Tea Res Inst, Kunming, Peoples R China

5.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing, Peoples R China

6.Hebei Gaoyi Stock Seed Farm, Gaoyi, Peoples R China

7.Univ Haifa, Inst Evolut, Haifa, Israel

8.Zhejiang Agr & Forestry Univ, Coll Adv Agr Sci, Linan, Peoples R China

9.Zhejiang Acad Agr Sci, Hangzhou, Peoples R China

10.Hainan Seed Ind Lab, Sanya, Peoples R China

期刊名称：NATURE GENETICS （ 影响因子：29.0； 五年影响因子：37.4 ）

ISSN： 1061-4036

年卷期： 2025 年 57 卷 6 期

页码：

收录情况： SCI

摘要： Resistance to wheat powdery mildew is commonly mediated by individual resistance proteins, most of which encode nucleotide-binding leucine-rich repeat (NLR) receptors. Here we report that the powdery mildew resistance gene MLIW170/PM26 in wild emmer and bread wheat derivatives is determined by a genetically linked atypical NLR pair TdCNL1/TdCNL5. Map-based cloning and PacBio HiFi long-read sequencing revealed that TdCNL1 encodes an atypical coiled-coil-domain-containing NLR protein (CNL) fused with a new potassium-dependent sodium-calcium exchanger integrated domain, whereas TdCNL5 encodes a canonical CNL protein. Mutagenesis and virus-induced gene silencing experiments indicated that both TdCNL1 and TdCNL5 are essential for powdery mildew resistance. Transgenic plants with TdCNL1 alone or TdCNL1/TdCNL5 together show resistance, whereas Fielder with TdCNL5 alone was susceptible. Geographically, MLIW170/PM26 occurs in a few Southern populations of wild emmer wheat. Our study highlights an atypical NLR pair coordinately regulating powdery mildew resistance and provides a diversified resistance gene resource for wheat improvement.