Development of nitrilase-mediated process for phenylacetic acid production from phenylacetonitrile
文献类型： 外文期刊
作者： Fan, Haiyang 1 ; Chen, Lifeng 1 ; Sun, Huihui 2 ; Wang, Hualei 1 ; Liu, Qinghai 1 ; Ren, Yuhong 1 ; Wei, Dongzhi 1 ;
作者机构： 1.East China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China
关键词： Biotransformation;Fed-batch reaction;Fermentation;Nitrilase;Phenylacetic acid
期刊名称：CHEMICAL PAPERS （ 影响因子：2.097； 五年影响因子：1.831 ）
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收录情况： SCI
摘要： This study aimed at developing an efficient biotransformation process for phenylacetic acid production from phenylacetonitrile by using recombinant Escherichia coli M15 harboring a double mutant MG nitrilase (I113M/Y199G) from Burkholderia cenocepacia J2315. A yield of 2310 U/mL nitrilase was obtained by fermentation after the optimization of cultivation conditions, with a specific activity of 64 U/mg dcw. The MG nitrilase showed high substrate tolerance and completely hydrolyzed 100 mM phenylacetonitrile in 30 min under optimal conditions. To alleviate substrate inhibition, periodic or continuous batch-feeding of substrate was used during the biotransformation. Up to 164 g/L substrate was completely hydrolyzed in 9 h with continuous batch-feeding using resting cells, corresponding to 400 U/mL of nitrilase activity, and leading to production of 163.4 g/L phenylacetic acid. The hydrolysis process has potential application for phenylacetic acid production on a large scale.
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