An UDP-Glucosyltransferase Gene from Barley Confers Disease Resistance to Fusarium Head Blight
文献类型： 外文期刊
作者： Xing, Li-Ping 1 ; He, Li-Qiang 1 ; Xiao, Jin 1 ; Chen, Qi-Guang 1 ; Li, Ming-Hao 2 ; Shang, Yi 3 ; Zhu, Yan-Fei 1 ; Chen, 1 ;
作者机构： 1.Nanjing Agr Univ, JCIC MCP, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China
2.Chinese Acad Sci, Hefei Inst Phys Sci, Hefei 230031, Peoples R China
3.Zh
关键词： UDP-glucosyltransferase gene;Hordeum vulgare L;Fusarium head blight (FHB);Deoxynivalenol (DON);In vitro antifungal activity;Virus-induced gene silencing (VIGS)
期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）
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收录情况： SCI
摘要： UDP-glucosyltransferases (UGTs) contribute to Fusarium head blight (FHB) resistance of wheat and barley by glycosylating the deoxynivalenol (DON), which is produced by Fusarium fungus. In this study, seven alleles of barley HvUGT14077 (GenBank No.GU170356.1) were cloned using RT-PCR. Among them, HvUGT-10W1, which was isolated from a FHB resistant barley variety 10W1, was significantly up-regulated in young spikes after F. graminearum (F.g) inoculation. HvUGT-10W1::GFP was subcellularly located in the plasma membrane and cytoplasm of the wheat protoplasts. In vitro antifungal activity assay showed that the HvUGT-10W1 protein exerted obvious inhibition against the growth of F.g. The silencing of the HvUGT-10W1 by virus-induced gene silencing (VIGS) resulted in compromised FHB resistance of 10W1, which was shown by the increased infected colonies on the leaves. These indicated that the barley HvUGT-10W1 may also contribute to F.g resistance in barley and provided a potential candidate gene to develop transgenic barley with enhanced FHB resistance.
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