文献类型： 外文期刊
作者： Goodman, Cynthia L. 1 ; Ringbauer, Joseph A., Jr. 1 ; Li, Yao-Fa 2 ; Lincoln, Tamra Reall 1 ; Stanley, David 1 ;
作者机构： 1.ARS, USDA, Biol Control Insects Res Lab, 1503 S Providence Rd, Columbia, MO 65203 USA
2.Hebei Acad Agr & Forestry Sci, Key Lab Integrated Pest Management Crops Northern, IPM Ctr Hebei Prov, Plant Protect Inst,Minist Agr, Baoding 07100, Peoples R China
关键词： Insect cell line;Hemiptera;Squash bug;Anasa tristis;DAF-PCR
期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL （ 影响因子：2.416； 五年影响因子：2.117 ）
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年卷期：
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收录情况： SCI
摘要： The squash bug, Anasa tristis, is a pest of cucurbits that exerts direct damage on crops and is a vector of plant pathogens. We established cell lines from this insect to serve as tools for basic biology, including virology and immunology, as well as applied studies, such as insecticide development programs. We initiated 15 cell cultures, using nine media or combinations of media. The media yielding the best results were a modification of Kimura's medium and a combination of two commercially available cell culture media (EX-CELL 420 and L15). We designated the two cell lines as BCIRL-AtE-CLG11 and BCIRL-AtE-CLG15. From the AtE-CLG15 line, we isolated two sub-lines, A and B. Of these, the most consistently replicating line was AtE-CLG15A. We determined the doubling time of this line (190 h) and its mean cell diameter (14.5 +/- 0.7 mu m). We characterized the AtE-CLG15A line using DAF-PCR. The BCIRL-AtE-CLG15A cell line is now available for researchers world-wide.
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