文献类型： 外文期刊
作者： Zhang, Hongwei 1 ; Li, Yaofa 3 ; Reall, Tamra 3 ; Xu, Yiyun 2 ; Goodman, Cynthia 3 ; Saathoff, Stephen 3 ; Ringbauer, 1 ;
作者机构： 1.Henan Inst Sci & Technol, Dept Entomol, Xinxiang 453007, Henan, Peoples R China
2.Univ Missouri Columbia, Div Plant Sci, Columbia, MO USA
3.USDA ARS, Biol Control Insects Res Lab, Columbia, MO USA
4.Hebei Acad Agr & Forestry Sci, IPM Ctr Hebei Prov, Plant Protect Inst,Minist Agr, Key Lab Integrated Pest Management Crops Northern, Baoding, Peoples R China
关键词： Spodoptera eridania; Cell line; Catalase
期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL （ 影响因子：2.416； 五年影响因子：2.117 ）
ISSN： 1071-2690
年卷期： 2018 年 54 卷 10 期
页码：
收录情况： SCI
摘要： Spodoptera eridania (southern armyworm) is a polyphagous pest of many plants, including field crops, vegetables, fruits, and ornamentals. Larvae are leaf feeders, defoliating many crops in the tropics and subtropics of the western hemisphere. In this study, cell lines from S. eridania were established to support research focused on the development of advanced pest management technologies. We generated seven cell lines from larval tissues: three from nervous tissues, two from testes, and two from fat bodies. These cell lines have been passaged 18-57 times, indicating they are established lines. They are maintained in EX-CELL 420 or a combination of L15 + EX-CELL 420 media. The identities of the cell lines were confirmed by DAF-PCR and their doubling times ranged from 42 to 110h. Microscopy indicated the presence of one or more morphologically distinct cell types in each cell line. We identified a catalase gene in all seven cell lines. H2O2 treatment suppressed the expression of catalase and led to a reduction in catalase activity. This is the first report of cell lines established from S. eridania, and these cell lines are now available to researchers worldwide on request.
- 相关文献
作者其他论文 更多>>
-
Indomethacin and 20-hydroxyecdysone influence protein expression in a Spodoptera frugiperda nervous system cell line
作者：Wang, Yong;Buer, Benjamin;Dohn, Susanne;Geibel, Sven;Goodman, Cynthia L.;Kang, David;Ringbauer, Joseph;Stanley, David;Reall, Tamra;Li, Yaofa;Kang, David
关键词：20-hydroxyecdysone; indomethacin; insect cell line; insect nervous system; lepidoptera; prostaglandin; Spodoptera frugiperda
-
Sublethal and transgenerational effects of insecticides used in whitefly control on biological traits of the parasitoid Eretmocerus hayati
作者：Guo, Mengran;Feng, Xueying;Yang, Kun;Wang, Lukuan;Xu, Haiyun;Guo, Mengran;Gao, Zhanlin;Li, Yaofa
关键词：parasitoid; insecticide; sublethal effect; transgenerational effect; life table
-
Detoxification enzyme is involved in the temperature effect on the toxicity of tetrachlorantraniliprole to Plutella xylostella
作者：An, Jingjie;Dang, Zhihong;Guo, Jianglong;Gao, Zhanlin;Li, Yaofa;Dou, Ya 'nan
关键词：Temperature effect; Insecticides; Plutella xylostella; Tetrachlorantraniliprole; Transcriptome analysis; Detoxification enzymes
-
Transcriptome analysis during vernalization in wheat (Triticum aestivum L.)
作者：Wang, Jiao;Sun, Lei;Zhang, Hongwei;Jiao, Bo;Wang, Haibo;Zhou, Shuo
关键词：Wheat (Triticum aestivum L; ); Vernalization; Shiluan02-1; Transcriptome analysis; RNA-seq
-
Genome-wide identification of genes encoding SWI/SNF components in soybean and the functional characterization of GmLFR1 in drought-stressed plants
作者：Chen, Qiang;Ai, Lijuan;Tian, Xuan;Zhang, Hongwei;Tian, Jiawang;Wang, Qianying;Cui, Sujuan;Zhao, Hongtao;Chen, Qiang;Shi, Xiaolei;Zhang, Mengchen;Yang, Chunyan
关键词：soybean; SWI; SNF components; GmLFR1; transgenic hairy root; drought stress
-
The Lethal and Sublethal Effects of Lambda-Cyhalothrin and Emamectin Benzoate on the Soybean Pest Riptortus pedestris (Fabricius)
作者：Guo, Jianglong;An, Jingjie;Li, Yaofa;Dang, Zhihong;Gao, Zhanlin;Chang, Hong;Wu, Chi
关键词：bean bug; bioassays; pyrethroid insecticide; avermectin family insecticide; two-sex life table; sublethal effects; vitellogenin gene
-
Identification of highly active compounds from insecticidal plant Oroxylum indicum L. (Vent.) and the induction of apoptosis by lapachol on Sf9 cells
作者：Zhao, Yujing;An, Jingjie;Dang, Zhihong;Guo, Jianglong;Gao, Zhanlin;Li, Yaofa;Zhao, Yujing;Ma, Shujie
关键词：Oroxylum indicum; Sf9 cells; Lapachol; Apoptosis; Aphid
