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Identification of highly active compounds from insecticidal plant Oroxylum indicum L. (Vent.) and the induction of apoptosis by lapachol on Sf9 cells

文献类型： 外文期刊

作者： Zhao, Yujing 1 ; An, Jingjie 1 ; Dang, Zhihong 1 ; Guo, Jianglong 1 ; Gao, Zhanlin 1 ; Ma, Shujie 2 ; Li, Yaofa 1 ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Plant Protect Inst, IPM Ctr Hebei Prov,Minist Agr & Rural Affairs, Key Lab Integrated Pest Management Crops Northern, Baoding 071000, Peoples R China

2.Hebei Agr Univ, Coll Plant Protect, Baoding 071001, Peoples R China

关键词： Oroxylum indicum; Sf9 cells; Lapachol; Apoptosis; Aphid

期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL （ 影响因子：2.1； 五年影响因子：2.1 ）

ISSN： 1071-2690

年卷期： 2023 年

页码：

收录情况： SCI

摘要： The extraction of biopesticides from plants has become a promising field for agricultural development. To explore a high-efficiency and viable method for the screening of plant compounds with insecticidal activity, we screened for active ingredients in the insecticidal plant, Oroxylum indicum L. Vent, using Sf9 cells. A CCK-8 cytotoxicity assay kit was used for high-throughput screening of 34 compounds contained in O. indicum. The apoptosis-inducing effect of the highly cytotoxic compound on Sf9 cells was investigated by morphological characterization using inverted microscopy, caspase-3 activity assay, and DNA gel electrophoresis. Finally, the biological activity of compounds against aphids was evaluated using the leaf-pest dipping methods and leaf dipping methods. Results showed that among the main compounds identified, lapachol, chrysin, and baicalein had good proliferation inhibitory effects on Sf9 cells, with their recorded IC50 being 11.53 mg/L, 38.39 mg/L, and 42.10 mg/L, respectively. Moreover, the IC50 value of lapachol was lower than the control insecticides rotenone (18.03 mg/L) and fipronil (21.04 mg/L). Apoptosis assay further showed that lapachol promoted the production of caspase-3 and led to DNA fragmentation in Sf9 cells. Lapachol showed high biological activity against Aphis gossypii, Sitobion avenae, and Semiaphis heraclei, with its recorded LC50 being 104.40, 101.80, and 110.29 mg/L, respectively, which were comparable to the activity of the control insecticide rotenone. High-throughput screening of active ingredients in the insecticidal plant O. indicum using Sf9 cells is feasible, and the identification of lapachol as the main aphidicidal active substance is valuable for further study.

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