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Prostaglandin actions in established insect cell lines

文献类型： 外文期刊

作者： Li, Yao-Fa 1 ; Zhang, Hongwei 1 ; Ringbauer, Joseph A., Jr. 1 ; Goodman, Cynthia L. 1 ; Lincoln, Tamra Reall 1 ; Zh 1 ;

作者机构： 1.ARS, Biol Control Insects Res Lab, USDA, 1503 S Providence Rd, Columbia, MO 65203 USA

2.Hebei Acad Agr & Forestry Sci, Key Lab Integrated Pest Management Crops Northern, IPM Ctr Hebei Prov, Plant Protect Inst,Minist Agr, Baoding 07100, Peoples R China

3.Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA

关键词： Insect cell lines;Coleoptera;Hemiptera;Lepidoptera;Prostaglandins;Eicosanoids;Inhibitors of eicosanoid biosynthesis

期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL （ 影响因子：2.416； 五年影响因子：2.117 ）

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收录情况： SCI

摘要： Prostaglandins (PGs) are oxygenated metabolites of arachidonic acid (AA) and two other C20 polyunsaturated fatty acids that serve as biochemical signals mediating physiological functions. We reported that PGs influence protein expression in insect cell lines, which prompted the question: do PGs influence cell proliferation or viability in insect cell lines? Here, we report on the outcomes of experiments designed to address the question in cell lines from three insect orders: Hemiptera (squash bug, Anasa tristis, BCIRL-AtE-CLG15A), Coleoptera (red flour beetle, Tribolium castaneum, BCIRL-TcA-CLG1), and Lepidoptera (tobacco budworm, Heliothis virescens, BCIRL-HvAM1). Treating the insect cell lines with PGA(1), PGA(2), or PGD(2) led to dose-dependent reductions in cell numbers. All three cell lines were sensitive to PGA(1) and PGA(2) (IC(50)s = 9.9 to 26.9 mu M) and were less sensitive to PGD(2) (IC(50)s = 31.6 to 104.7 mu M). PG treatments also led to cell death at higher concentrations, as seen in mammalian cell lines. PGE(1), PGE(2), and PGF(2 alpha) treatments did not influence AtE-CLG15A or HvAM1 cell numbers at lower concentrations, but led to dose-related reductions in TcA-CLG1 cells at higher concentrations. Similar treatments with pharmaceutical inhibitors of PG biosynthesis also led to reduced cell numbers: MAFP (inhibits phospholipase A(2)), indomethacin (inhibits PG biosynthesis), and esculetin (inhibits lipoxygenase). Because these pharmaceuticals are used to relieve inflammation and other medical issues in human medicine, they are not toxic to animal cells. We infer PGs are necessary in optimal quantities for ongoing homeostatic functions in established cell lines; in quantities outside the optimal concentrations, PGs are deleterious.

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[1]Cell lines derived from the squash bug, Anasa tristis (Coreidae: Hemiptera). Goodman, Cynthia L.,Ringbauer, Joseph A., Jr.,Lincoln, Tamra Reall,Stanley, David,Li, Yao-Fa.

[2]Effects of the Cry1Ac toxin of Bacillus thuringiensis on Microplitis mediator, a parasitoid of the cotton bollworm, Helicoverpa armigera. Liu, XX,Zhang, QW,Zhao, JZ,Cai, QN,Xu, HL,Li, JC. 2005

[3]Seasonal cues mediate host behaviour modification and cocoon polymorphism in Microplitis mediator (Hymenoptera: Braconidae). Zhang, Jing,Liu, Xiao-Xia,Zhang, Qing-Wen,Michaud, J. P.,Li, Jian-Cheng,Luo, Shu-Ping.

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