您好,欢迎访问中国水产科学研究院 机构知识库!

Experimental inoculation of oriental river prawn Macrobrachium nipponense with white spot syndrome virus (WSSV)

文献类型: 外文期刊

作者: Zhao, Caiyuan 1 ; Fu, Hongtuo 1 ; Sun, Shengming 2 ; Qiao, Hui 2 ; Zhang, Wenyi 2 ; Jin, Shubo 2 ; Jiang, Sufei 3 ; Xiong 1 ;

作者机构: 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214081, Peoples R China

2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi 214081, Peoples R China

3.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi 214081, Peoples R

关键词: Nimaviridae;Whispovirus;Crustacean;LD50;Histopathology

期刊名称:DISEASES OF AQUATIC ORGANISMS ( 影响因子:1.802; 五年影响因子:2.148 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: The oriental river prawn Macrobrachium nipponense is an economically important species that is widely farmed in China. White spot syndrome virus (WSSV) is one of the most devastating pathogens of the cultured shrimp Litopenaeus vannamei, responsible for massive loss of its commercial products worldwide. We investigated the infectivity and pathogenicity of WSSV in adult M. nipponense using standardized conditions for L. vannamei. The median lethal dose of WSSV in adult M. nipponense was 10(3.84 +/- 0.06) copies g(-1), which was about 1000-fold higher than in L. vannamei (10(0.59 +/- 0.22) copies g(-1)). WSSV was detected by 2-step PCR in the gills, hepatopancreas, muscle, stomach, heart, gut, nerve, integument, pereopod, eyestalk, testis, and ovary of experimentally infected dead M. nipponense. Lesions were observed histologically following WSSV injection, showing basophilic intranuclear inclusion bodies in the hepatopancreas and subsequently in the gills. The clearance of WSSV was observed in hepatopancreas and gills at 48 and 96 h post-inoculation, respectively. No histological lesions were detected in muscle from 0-96 h post-injection. The results show that the oriental river prawn M. nipponense can be infected by WSSV and the infections are self limiting over time; therefore, M. nipponense may serve as a useful model for studying resistance to WSSV.

  • 相关文献

[1]三疣梭子蟹“黄选1号”盐度耐受性分析. 隋延鸣,高保全,刘萍,任宪云,李洋. 2012

[2]3株O3血清型鳗弧菌灭活疫苗的免疫原性和免疫保护效果. 李淑芳,唐磊,李杰,李贵阳,王晓冉,王小鹿,丁山,莫照兰. 2018

[3]Molecular cloning and expression analysis of Fem1b from oriental river prawn Macrobrachium nipponense. Rahman, N. M. A.,Fu, H.,Bai, H.,Jiang, F. W.,Liang, G.,Jiang, F. F.,Wu, Y.,Fu, H.,Qiao, H.,Jin, S.,Zhang, W.,Sun, S.,Gong, Y.,Xiong, Y.,Rahman, N. M. A.. 2016

[4]Six chitinases from oriental river prawn Macrobrachium nipponense: cDNA characterization, classification and mRNA expression during post-embryonic development and moulting cycle. Zhang, Shiyong,Fu, Hongtuo,Jiang, Fengwei,Jin, Shubo,Jiang, Sufei,Xiong, Yiwei,Fu, Hongtuo,Sun, Shengming,Qiao, Hui,Zhang, Wenyi,Jin, Shubo,Gong, Yongsheng. 2014

[5]cDNA cloning and expression of Ubc9 in the developing embryo and ovary of oriental river prawn, Macrobrachium nipponense. Zhang, Fengying,Chen, Liqiao,Wu, Ping,Zhao, Weihong,Li, Erchao,Zhang, Fengying,Qin, Jianguang.

[6]Molecular cloning of two tropomyosin family genes and expression analysis during development in oriental river prawn, Macrobrachium nipponense. Jin, Shubo,Fu, Hongtuo,Jin, Shubo,Jiang, Sufei,Xiong, Yiwei,Qiao, Hui,Sun, Shengming,Zhang, Wenyi,Li, Fajun,Gong, Yongsheng,Fu, Hongtuo.

[7]Two transcripts of HMG-CoA reductase related with developmental regulation from Scylla paramamosain: Evidences from cDNA cloning and expression analysis. Zhao, Ming,Jiang, Keji,Song, Wei,Ma, Chunyan,Wang, Jing,Meng, Yongyong,Wei, Hongqing,Qiao, Zhenguo,Zhang, Fengying,Ma, Lingbo,Zhao, Ming,Wang, Jing,Meng, Yongyong,Wei, Hongqing,Chen, Kai.

[8]Molecular cloning and expression analysis of two sex-lethal homolog genes during development in the oriental river prawn, Macrobrachium nipponense. Zhang, Y. P.,Zhang, W. Y.,Fu, H. T.,Zhang, Y. P.,Qiao, H.,Sun, S. M.,Jiang, S. F.,Gong, Y. S.,Xiong, Y. W.,Jin, S. B.,Fu, H. T.. 2013

[9]Characterization of the male-specific lethal 3 gene in the oriental river prawn, Macrobrachium nipponense. Zhang, Y. P.,Sun, S. M.,Fu, H. T.,Ge, X. P.,Qiao, H.,Zhang, W. Y.,Xiong, Y. W.,Jiang, S. F.,Gong, Y. S.,Jin, S. B.. 2015

[10]Physiological responses and gene expression changes in the western mosquitofish (Gambusia affinis) exposed to progesterone at environmentally relevant concentrations. Hou, Liping,Yang, Yang,Shu, Hu,Cheng, Xuemei,Xu, Hongyan,Ying, Guangguo,Zhao, Jianliang,Ying, Guangguo,Zhao, Jianliang. 2017

[11]Edwardsiellosis in farmed turbot, Scophthalmus maximus (L.), associated with an unusual variant of Edwardsiella tarda: a clinical, aetiological and histopathological study. Qin, L.,Xu, J.,Wang, Y. G.. 2014

[12]Histopathological Liver and Testis Alterations in Male Half Smooth Tongue Sole (Cynoglossus semilaevis) Exposed to Endocrine Disruptors. Li, Fengling,Yao, Lin,Sun, Weihong,Jiang, Yanhua,Li, Zhaoxin,Zhai, Yuxiu. 2017

[13]Recovery and pathogenicity analysis of Aerococcus viridans isolated from tilapia (Orecohromis niloticus) cultured in southwest of China. Ke, Xiaoli,Lu, Maixin,Ye, Xing,Gao, Fengying,Zhu, Huaping,Huang, Zhanghan. 2012

[14]Isolation, pathogenicity and characterization of a novel bacterial pathogen Streptococcus uberis from diseased mandarin fish Siniperca chuatsi. Luo, Xia,Fu, Xiaozhe,Liao, Guoli,Chang, Ouqin,Huang, Zhibin,Li, Ningqiu,Fu, Xiaozhe,Li, Ningqiu.

[15]Thelohanellus wangi n. sp (Myxozoa, Myxosporea), a new gill parasite of allogynogenetic gibel carp (Carassius auratus gibelio Bloch) in China, causing severe gill myxosporidiosis. Yuan, S.,Wang, J. G.,Zhang, J. Y.,Yuan, S.,Xi, B. W.,Xie, J..

[16]Susceptibility of farmed juvenile giant grouper Epinephelus lanceolatus to a newly isolated grouper iridovirus (genus Ranavirus). Peng, Chao,Ma, Hongling,Su, Youlu,Wen, Weigeng,Feng, Juan,Guo, Zhixun,Qiu, Lihua,Peng, Chao.

作者其他论文 更多>>