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Expression and Purification of a Functional Porcine Soluble Triggering Receptor Expressed on Myeloid Cells 1

文献类型： 外文期刊

作者： Li, Hui 1 ; Guo, Shuangshuang 2 ; Yan, Leyan 1 ; Meng, Chunhua 1 ; Hu, Yiyi 3 ; He, Kongwang 3 ; Shi, Zhendan 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Anim Sci, Key Lab Anim Breeding & Reprod, Room 515, Nanjing, Jiangsu, Peoples R China

2.Shandong Agr Univ, Coll Anim Sci & Vet Med, Tai An, Shandong, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing, Jiangsu, Peoples R China

关键词： Cytokines;Inflammatory responses;LPS;Porcine;TREM-1

期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：2.282； 五年影响因子：1.702 ）

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收录情况： SCI

摘要： Triggering receptor expressed on myeloid cells 1 (TREM-1) plays a vital role in the pathogen-triggered amplification loop required for proinflammatory responses. Blockade of TREM-1 signaling may inhibit expansion of sepsis and prolong survival of animals. In the present study, the gene of porcine soluble TREM-1 was cloned and expressed in E. coli. After purification, the bioactivity of recombinant porcine soluble TREM-1 was tested in vitro on porcine alveolar macrophages. The results showed that supplementation with the recombinant porcine sTREM-1 protein rapidly and dose-dependently attenuated the upregulation of cytokines (IL-1, IL-2, IL-4, IL-8, IL-10, IL-12, IL-16, IL-18, and TNF-) caused by LPS stimulation in the cultured porcine alveolar macrophages. These results indicate that the recombinant porcine sTREM-1 protein can prevent TREM-1-mediated hyperinflammatory responses after exposure to LPS.

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