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Analyses of long non-coding RNAs and mRNA profiling through RNA sequencing of MDBK cells at different stages of bovine viral diarrhea virus infection

文献类型： 外文期刊

作者： Ma, Qiman 1 ; Li, Liangyuan 2 ; Tang, Yan 5 ; Fu, Qiang 4 ; Liu, Sheng 2 ; Hu, Shengwei 3 ; Qiao, Jun 2 ; Chen, Chuangfu; 1 ;

作者机构： 1.Shihezi Univ, Coll Med, Shihezi 832003, Xinjiang, Peoples R China

2.Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832003, Xinjiang, Peoples R China

3.Shihezi Univ, Coll Life Technol, Shihezi 832003, Xinjiang, Peoples R China

4.Xinjiang Agr Univ, Coll Vet Med, Urumqi 830052, Xinjiang, Peoples R China

5.Xinjiang Agr Vocat Tech Coll, Anim Sci & Technol Branch, Changji 831100, Xinjiang, Peoples R China

6.Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi 832000, Xinjiang, Peoples R China

关键词： Long non-coding RNA (lncRNA);Cellular gene expression;BVDV infection;RNA sequencing;lncRNA expression profile;Functional enrichment

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.534； 五年影响因子：2.382 ）

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收录情况： SCI

摘要： AbstractBackgroundBovine viral diarrhea virus (BVDV) infection is a dynamic and complex process that leads to significant economic losses in the dairy and cattle industries. However, our understanding of the protective and pathological mechanism underlying host infection is limited.MethodsTo determine whether BVDV regulates specific activities of the host cell, the expression of long non-coding RNA (lncRNA) during BVDV NADL infection was studied by deep sequencing.ResultsA total of 1236 lncRNA transcripts and 3261 mRNA transcripts were differentially regulated at 2h, 6h, and 18h post-infection. The lncRNAs shared same characteristics with other mammals in terms of exon length, number, expression level, and conservation. The Gene Ontology (GO) enrichment and KEGG pathway analyses showed that lncRNAs regulate immune reaction during BVDV infection. Thirteen differentially expressed genes in 18 hpi were selected and independently validated by reverse-transcription qPCR.ConclusionsThe present study is the first to provide insights into the biological connection of lncRNAs and BVDV, which can be further explored for the development of antiviral prevention strategies and in understanding persistent infection between viral and host components.Highlights?A lot of differentially expressed lncRNAs and mRNAs were found post BVDV infection.?Enrichment function shows that lncRNAs regulate immune response.?The results of RNA-seq were validated by RT-qPCR.]]>

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