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The secretory pathway and the actomyosin motility system are required for plasmodesmatal localization of the P7-1 of rice black-streaked dwarf virus

文献类型： 外文期刊

作者： Sun, Zongtao 1 ; Zhang, Shanglin 2 ; Xie, Li 1 ; Zhu, Qisong 3 ; Tan, Zilong 4 ; Bian, Jing 4 ; Sun, Liying 1 ; Chen, Jianping 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Key Lab Biotechnol Plant Protect, Minist Agr,Inst Virol & Biotechnol, Hangzhou 310021, Zhejiang, Peoples R China

2.Anhui Agr Univ, Coll Plant Protect, Hefei 230036, Peoples R China

3.Shandong Rice Res Inst, Jinan 250100, Peoples R China

4.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Peoples R China

期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

ISSN： 0304-8608

年卷期： 2013 年 158 卷 5 期

页码：

收录情况： SCI

摘要： Rice black-streaked dwarf virus (RBSDV), a plant-infecting reovirus (genus Fijivirus), generally induces virus-containing tubules in infected cells. The nonstructural protein P7-1, encoded by the first open reading frame of segment 7, is involved in forming the structural matrix of these tubules. In experiments to investigate the subcellular localization of P7-1 in Nicotiana benthamiana epidermal cells, fluorescence of P7-1:eGFP was observed in the nucleus, cytoplasm and cell periphery, and in punctate points along the cell wall of plasmolyzed cells. Co-localization with plasmodesmata-located protein 1 showed that P7-1 formed the punctate points at plasmodesmata. Mutational analysis demonstrated that transmembrane domain 1 and adjacent residues were necessary and sufficient for P7-1 to form punctate structures at the cell wall in the plasmolyzed cells. Chemical drug and protein inhibitor treatments indicated that P7-1 utilized the ER-to-Golgi secretory pathway and the actomyosin motility system for its intracellular transport. The plasmodesmatal localization of RBSDV P7-1 is therefore dependent on the secretory pathway and the actomyosin motility system.

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