文献类型： 外文期刊

作者： Cheng, Jie ¹ ; Wei, Fan ¹ ; Zhang, Mingfei ² ; Li, Nan ² ; Song, Tianqi ¹ ; Wang, Yong ¹ ; Chen, Dongsheng ³ ; Xiang, Jis ¹ ;

作者机构： 1.Northwest A&F Univ, Coll Agron, Yangling 712100, Shaanxi, Peoples R China

2.Chi Feng Univ, Acad Agr Sci, Key Lab Agroecol Protect & Exploitat & Utilizat A, Chifeng, Peoples R China

3.Ningxia Acad Agr & Forestry Sci, Crop Res Inst, Yinchuan 750002, Ningxia, Peoples R China

关键词： Common wheat; TaNRX1-D promoter; Osmotic or ABA stress; Cis-regulatory element

期刊名称：GENES & GENOMICS （ 影响因子：1.839； 五年影响因子：1.329 ）

ISSN： 1976-9571

年卷期： 2021 年 43 卷 9 期

页码：

收录情况： SCI

摘要： Background Cloning and characterizing the drought-inducible promoters is essential for their use in crop resistance's genetic improvement. Previous studies have shown that the TaNRX1-D gene participates in regulating the response of wheat to drought stress. However, its promoter has not yet been identified. Objective In this study, we aimed to characterize the promoter of the TaNRX1-D gene. Methods The promoter of TaNRX1-D (named P0, 2081 bp) was isolated from common wheat with several cis-acting elements that regulate in response to abiotic stresses and some core cis-acting elements. Functional verification of the promoter, eight 5'-deletion fragments of TaNRX1-D promoter, was fused to the beta-glucuronidase (GUS) gene P0::GUS similar to P7::GUS and transformed into Arabidopsis, respectively. Agrobacterium-mediated GUS transient assay the P6a and P6b promoter regions in tobacco leaves under normal, osmotic or ABA stress. Results Activity analysis of the full-length promoter (P0) showed that the intensity of stronger beta-glucuronidase (GUS) staining in the roots and leaves was obtained during the growth of transgenic Arabidopsis. P0::GUS displayed the GUS activity was much higher in the roots and leaves than in other parts of the transgenic plant under normal conditions, which was similarly within wheat. Analysis of the 5'-deletion fragments revealed that P0::GUS similar to P6::GUS responded well upon exposure to osmotic (polyethylene glycol-6000, PEG6000) and abscisic acid (ABA) stress treatments and expressed significantly higher GUS activity than the CaMV35S promoter ( 35S::GUS), while P7:: GUS did not. GUS transient assay in tobacco leaves showed that the GUS activities of P6a and P6b were lower than P6 in the PEG6000 and ABA stresses. Conclusion The 193 bp (P6) segment was considered the core region of TaNRX1-D responding to PEG6000 or ABA treatment. GUS activity assay in transgenic Arabidopsis showed that this segment was sufficient for the PEG6000 or ABA stress response. The identified 193 bp promoter of TaNRX1-D in this study will help breed osmotic or ABA tolerant crops. The 36 bp segment between P6 and P6b (-193 to -157 bp) was considered the critical sequence for the TaNRX1-D gene responding to PEG6000 or ABA treatment.