您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Expression analysis and antiviral activity of koi carp (Cyprinus carpio) viperin against carp edema virus (CEV)

文献类型： 外文期刊

作者： Wang, Xiao-wen 1 ; Zhang, Rong 1 ; Liu, Li-li 1 ; Li, Hui-juan 1 ; Zhu, Hua 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Key Lab Fishery Biotechnol, Beijing 100068, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Fisheries Res Inst, Beijing 100068, Peoples R China

3.Natl Freshwater Fisheries Engn Technol Res Ctr, Beijing 100068, Peoples R China

关键词： Viperin; koi carp ( Cyprinus carpio ); Carp edema virus (CEV); Antiviral activity; Immune regulation

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2024 年 148 卷

页码：

收录情况： SCI

摘要： Viperin, also known as radical S-Adenosyl methionine domain containing 2 (RSAD2), is an IFN stimulated protein that plays crucial roles in innate immunity. Here, we identified a viperin gene from the koi carp (Cyprinus carpio) (kVip). The ORF of kVip is 1047 bp in length, encoding a polypeptide of 348 amino acids with neither signal peptide nor transmembrane protein. The predicted molecular weight is 40.37 kDa and the isoelectric point is 7.7. Multiple sequence alignment indicated that putative kVip contains a radical SAM superfamily domain and a conserved C-terminal region. kVip was highly expressed in the skin and spleen of healthy koi carps, and significantly stimulated in both natural and artificial CEV-infected koi carps. In vitro immune stimulation analysis showed that both extracellular and intracellular poly (I: C) or poly (dA: dT) caused a significant increase in kVip expression of spleen cells. Furthermore, intraperitoneal injection of recombinant kVip (rkVip) not only reduced the CEV load in the gills, but also improved the survival of koi carps following CEV challenge. Additionally, rkVip administration effectively regulated inflammatory and anti-inflammatory cytokines (IL-6, IL-1 beta, TNF-alpha, IL-10) and interferon-related molecules (cGAS, STING, MyD88, IFN-gamma, IFN-alpha, IRF3 and IRF9). Collectively, kVip effectively responded to CEV infection and exerted antiviral function against CEV partially by regulation of inflammatory and interferon responses.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台