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Isolation of monocrotophos-degrading strain Sphingobium sp YW16 and cloning of its TnopdA

文献类型： 外文期刊

作者： Sun, Lina 1 ; Liu, Hongming 3 ; Gao, Xinhua 1 ; Chen, Wei 1 ; Huang, Kaihua 1 ; Zhang, Sui 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Ecoenvironm Protect Res Inst, Shanghai 201403, Peoples R China

2.Shanghai Engn Res Ctr Low Carbon Agr SERCLA, Shanghai 201403, Peoples R China

3.Anhui Normal Univ, Inst Mol Biol & Biotechnol, Wuhu 241000, Peoples R China

关键词： Monocrotophos;Biodegradation;Sphingobium sp YW16;TnopdA

期刊名称：ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH （ 影响因子：4.223； 五年影响因子：4.306 ）

ISSN： 0944-1344

年卷期： 2018 年 25 卷 5 期

页码：

收录情况： SCI

摘要： The bacterial strain Sphingobium sp. YW16, which is capable of degrading monocrotophos, was isolated from paddy soil in China. Strain YW16 could hydrolyze monocrotophos to dimethylphosphate and N-methylacetoacetamide and utilize dimethylphosphate as the sole carbon source but could not utilize N-methylacetoacetamide. Strain YW16 also had the ability to hydrolyze other organophosphate pesticides. A fragment (7067 bp) that included the organophosphorus hydrolase gene, opdA, was acquired from strain YW16 using the shotgun technique combined with SEFA-PCR. Its sequence illustrated that opdA was included in TnopdA, which consisted of a transpose gene, a putative integrase gene, a putative ATP-binding protein gene, and opdA. Additionally, a conjugal transfer protein gene, traI, was located downstream of TnopdA. The juxtaposition of TnopdA with TraI suggests that opdA may be transferred from strain YW16 to other bacteria through conjugation. OpdA was able to hydrolyze a wide range of organophosphate pesticides, with the hydrolysis efficiency decreasing as follows: methyl parathion > fenitrothion > phoxim > dichlorvos > ethyl parathion > trichlorfon > triazophos > chlorpyrifos > monocrotophos > diazinon. This work provides the first report of opdA in the genus Sphingobium.

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