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Knockdown of a cellulose synthase gene BoiCesA affects the leaf anatomy, cellulose content and salt tolerance in broccoli

文献类型： 外文期刊

作者： Li, Shuangtao 1 ; Zhang, Lei 1 ; Wang, Ying 1 ; Xu, Fengfeng 1 ; Liu, Mengyun 1 ; Lin, Peng 1 ; Ren, Shuxin 3 ; Ma, Rui 4 ;

作者机构： 1.China Agr Univ, Coll Hort, Beijing 100193, Peoples R China

2.Shanghai Acad Agr Sci, Inst Hort Res, Shanghai 201403, Peoples R China

3.Virginia State Univ, Sch Agr, POB 9061, Petersburg, VA 23806 USA

4.Jilin Acad Agr Sci, Agrobiotechnol Res Inst, Changchun 130033, Peoples R China

期刊名称：SCIENTIFIC REPORTS （ 影响因子：4.379； 五年影响因子：5.133 ）

ISSN： 2045-2322

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： Cellulose is the major component of cell wall materials. A 300 bp specific fragment from the cDNA fragment was chosen to insert into vector pFGC1008 at forward and reverse orientations to construct the recombinant RNAi vector. Knockdown of BoiCesA caused "dwarf" phenotype with smaller leaves and a loss of the content of cellulose. Moreover, RT-PCR analysis confirmed that the expression of the RNAi apparatus could repress expression of the CesA gene. Meanwhile, examination of the leaves from the T3 of RNAi transformants indicated reduction of cell expansion in vascular bundles, particularly on their abaxial surface. The proline and soluble sugar content increased contrarily. Under the salt stress, the T3 of RNAi plants showed significant higher resistance. The expression levels of some salt tolerance related genes (BoiProH, BoiPIP2; 2, BoiPIP2; 3) were significantly changed in T3 of RNAi plants. The results showed that the hairpin structure of CesA specific fragment inhibited the endogenous gene expression and it was proved that the cDNA fragment was relevant to the cellulose biosynthesis. Moreover, modulation cellulose synthesis probably was an important influencing factor in polysaccharide metabolism and adaptations of plants to stresses. This will provide technological possibilities for the further study of modulation of the cellulose content of crops.

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