文献类型： 外文期刊

作者： Liu, Qing ¹ ; Shen, Gezhi ² ; Peng, Keqin ¹ ; Huang, Zhigang ¹ ; Tong, Jianhua ¹ ; Kabir, Mohammed Humayun ¹ ; Wang, J ¹ ;

作者机构： 1.Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Peoples R China

2.Shanghai Acad Agr Sci, Crop Inst, Shanghai 201106, Peoples R China

3.Sichuan Acad Agr Sci, Inst Hort, Chengdu 610066, Peoples R China

4.Pe

关键词： Oryza sativa;OsmiR156f;plant architecture;protease inhibitor;T-DNA insertion

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2015 年 57 卷 10 期

页码：

收录情况： SCI

摘要： Plant architecture is an important factor for crop production. Some members of microRNA156 (miR156) and their target genes SQUAMOSAPromoter-Binding Protein-Like (SPL) were identified to play essential roles in the establishment of plant architecture. However, the roles and regulation of miR156 is not well understood yet. Here, we identified a T-DNA insertion mutant Osmtd1 (Oryza sativa multi-tillering and dwarf mutant). Osmtd1 produced more tillers and displayed short stature phenotype. We determined that the dramatic morphological changes were caused by a single T-DNA insertion in Osmtd1. Further analysis revealed that the T-DNA insertion was located in the gene Os08g34258 encoding a putative inhibitor I family protein. Os08g34258 was knocked out and OsmiR156f was significantly upregulated in Osmtd1. Overexpression of Os08g34258 in Osmtd1 complemented the defects of the mutant architecture, while overexpression of OsmiR156f in wild-type rice phenocopied Osmtd1. We showed that the expression of OsSPL3, OsSPL12, and OsSPL14 were significantly downregulated in Osmtd1 or OsmiR156f overexpressed lines, indicating that OsSPL3, OsSPL12, and OsSPL14 were possibly direct target genes of OsmiR156f. Our results suggested that OsmiR156f controlled plant architecture by mediating plant stature and tiller outgrowth and may be regulated by an unknown protease inhibitor I family protein.