文献类型： 外文期刊

作者： Chu, Y. X. ¹ ; Chen, H. R. ¹ ; Wu, A. Z. ¹ ; Cai, R. ² ; Pan, J. S. ² ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Agri Food Stand & Testing Technol, Shanghai, Peoples R China

2.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200030, Peoples R China

关键词： Calibrachoa hybrida;Rosa chinensis;Petunia hybrida;Dihydroflavonol 4-reductase genes

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 2 期

页码：

收录情况： SCI

摘要： Dihydroflavonol 4-reductase (DFR) genes from Rosa chinensis (Asn type) and Calibrachoa hybrida (Asp type), driven by a CaMV 35S promoter, were integrated into the petunia (Petunia hybrida) cultivar 9702. Exogenous DFR gene expression characteristics were similar to flower-color changes, and effects on anthocyanin concentration were observed in both types of DFR gene transformants. Expression analysis showed that exogenous DFR genes were expressed in all of the tissues, but the expression levels were significantly different. However, both of them exhibited a high expression level in petals that were starting to open. The introgression of DFR genes may significantly change DFR enzyme activity. Anthocyanin ultra-performance liquid chromatography results showed that anthocyanin concentrations changed according to DFR enzyme activity. Therefore, the change in flower color was probably the result of a DFR enzyme change. Pelargonidin 3-O-glucoside was found in two different transgenic petunias, indicating that both CaDFR and RoDFR could catalyze dihydrokaempferol. Our results also suggest that transgenic petunias with DFR gene of Asp type could biosynthesize pelargonidin 3-O-glucoside.