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Multiplex Lateral Flow Immunoassay for Mycotoxin Determination

文献类型： 外文期刊

作者： Song, Suquan 1 ; Liu, Na 1 ; Zhao, Zhiyong 1 ; Ediage, Emmanuel Njumbe 2 ; Wu, Songling 3 ; Sun, Changpo 3 ; De Saeger 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Minist Agr, Lab Qual & Safety Risk Assessment Agroprod Shangh, Inst Agrofood Stand & Testing Technol, Shanghai 201403, Peoples R China

2.Univ Ghent, Lab Food Anal, B-9000 Ghent, Belgium

3.Acad State Adm Grain PRC, Beijing 100037, Peoples R China

期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：6.986； 五年影响因子：6.755 ）

ISSN： 0003-2700

年卷期： 2014 年 86 卷 10 期

页码：

收录情况： SCI

摘要： A new lateral flow immunoassay (LFA) is proposed for qualitative and/or semiquantitative determination of aflatoxin B1 (AFB1), zearalenone (ZEA), deoxynivalenol (DON), and their analogues (AFs, ZEAs, DONs) in cereal samples. Each of the mycotoxin specific antibody was class specific and there was no cross reactivity to other groups of compounds. The visual limits of detection (vLOD) of the strip were 0.03, 1.6, and 10 mu g/kg for AFB1, ZEA and DON, respectively. The calculated limits of detection (cLOD) were 0.05, 1, and 3 mu g/kg respectively. Meanwhile the cutoff values were achieved at 1, 50, and 60 mu g/kg for AFB1, ZEA and DON, respectively. Recoveries ranged from 80% to 122% and RSD from 5% to 20%. Both the vLOD and CLOD for the three mycotoxins were lower than the EU maximum levels. Analysis of naturally contaminated maize samples resulted in a good agreement between the multiplex LFA and LC-MS/MS (100% for DONs and AFs, and 81% for ZEAs). Careful analysis of the results further explained the general overestimation of LFA compared to chromatographic methods for quantification of mycotoxins.

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