文献类型： 外文期刊

作者： Sun, Yi ¹ ; Huang, He ¹ ; Meng, Li ¹ ; Hu, Ke ¹ ; Dai, Si-Lan ¹ ;

作者机构： 1.Beijing Forestry Univ, Coll Landscape Architecture, Beijing 100083, Peoples R China

2.Shanghai Acad Agr Sci, Forestry & Fruit Res Inst, Shanghai 201403, Peoples R China

3.Life & Med Sci Higher Educ Press, Beijing 100029, Peoples R China

期刊名称：PHYSIOLOGIA PLANTARUM （ 影响因子：4.5； 五年影响因子：4.576 ）

ISSN： 0031-9317

年卷期： 2013 年 149 卷 2 期

页码：

收录情况： SCI

摘要： As the key enzyme in the biosynthesis of blue flower color pigments, flavonoid 3,5-hydroxylase (F35H) can catalyze the conversion of its major substrates, 2-S naringenin and dihydrokaempferol, into 3,4,5-hydroxylated pentahydroxyflavanone and dihydromyricetin, respectively. Unlike other F35Hs belonging to the CYP75A subfamily, Asteraceae-specific F35Hs belong to the CYP75B subfamily. Furthermore, cineraria F35H expressed in yeast exhibited not only F3H (flavonoid 3-hydroxylase) activity but also F35H activity in vitro. In this study, Southern blotting showed that there was only one copy of a homolog of the F35H gene PCFH in the Pericallisxhybrida genome. This gene could be detected by Northern blot in the primary developmental stages of ligulate florets of the purple- and blue-flowered cultivars, and its transcripts also accumulated in the leaves. Heterologous expression of PCFH could produce new delphinidin derivatives in the corollas of transgenic tobacco plants, increased the content of cyanidin derivatives and lead to the blue- and red-shifting of flower color in T-0 generation plants. These results indicate that cineraria F35H exhibited both F35H- and F3H-activity in vivo. The types and contents of anthocyanins and flower color phenotypes of the T-1 generation were similar to those of T-0 generation plants. PCFH exhibited stable inheritance and normal functions between generations. This study supplies new evidence to understand Asteraceae-specific F35Hs and provides important references for the further study of molecular breeding of blue-flowered chrysanthemums using the PCFH gene.