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DEVELOPMENT OF PLANT REGENERATION SYSTEM VIA SOMATIC EMBRYOGENESIS FROM ROOTS OF LILIUM HYBRID CULTIVARS

文献类型： 外文期刊

作者： Zhou, Yin 1 ; Zhang, Jianjun 1 ; Chen, Minmin 1 ; Gu, Junjie 2 ;

作者机构： 1.Shanghai Acad Agr Sci, Forestry & Pomol Res Inst, Shanghai 201403, Peoples R China

2.Shanghai Flower Port Enterprise Dev Co Ltd, Shanghai 201303, Peoples R China

关键词： cut flower;embryoids;embryogenic calluses;monocotyledonous;picloram;root system

期刊名称：PROPAGATION OF ORNAMENTAL PLANTS （ 影响因子：0.281； 五年影响因子：0.453 ）

ISSN： 1311-9109

年卷期： 2013 年 13 卷 3 期

页码：

收录情况： SCI

摘要： A simple and efficient protocol for in vitro plant regeneration through somatic embryogenesis was developed in four Lilium cultivars. Roots from in vitro seedlings of four lily cultivars were cultured on Murashige and Skoog (MS) medium (1962) supplemented with the auxin picloram (PIC) used alone (0.5, 1.0, and 2.0 mg l(-1)) or in combination with the cytokinins BAP or TDZ at 0.5 mg l(-1). Picloram with the combination of cytokinins was found to be positive to improve the callus formation. Generally speaking MS medium supplemented with 1.0 mg l(-1) PIC and 0.5 mg l(-1) BAP gave the highest percentage of explants forming callus in three Lilium cultivars ('Kankado' = 95.7%; 'Manissa' = 98%; 'Siberia' = 80%); for 'Sorbonne' the best performance for callus formation was scored on MS medium + 1.0 mg l(-1) PIC and 0.5 mg l(-1) TDZ (88.7%). Embryoids regeneration occurred after a subsequent transfer onto fresh medium and a further one month of culture. The number of embryoids per 0.1 g of callus ranged from 11.7 ('Siberia' on MS medium + 1.0 mg l(-1) PIC and 0.5 mg l(-1) TDZ) to 70.3 ('Manissa' on MS medium + 1.0 mg l(-1) PIC and 0.5 mg l(-1) BAP). The callus induction efficiency, callus growth index, callus growth intensity, and numbers of embryoids induced from 0.1 g of callus of root were higher than that of bulb scales and leaves. Embryoids cultured on MS medium with 0.1 mg l(-1) CPPU developed into complete plants.

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