Ontogenic Expression Pattern and Genetic Polymorphism of the Peroxisome Proliferator-Activated Receptor (PPAR-gamma) Gene in Goose Populations
文献类型: 外文期刊
作者: Wu, Hua-Li 1 ; Wang, Hui-Ying 1 ; He, Da-Qian 1 ; Liu, Yi 1 ; Gong, Shao-Ming 1 ; Kong, Fan-Li 2 ; Zhu, Qing 2 ;
作者机构: 1.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Shanghai 201106, Peoples R China
2.Sichuan Agr Univ, Coll Anim Sci & Technol, Lab Anim Genet & Breeding, Yaan 625014, Sichuan, Peoples R China
关键词: Goose;PPAR-gamma gene;SNP;carcass traits;expression
期刊名称:JOURNAL OF ANIMAL AND VETERINARY ADVANCES ( 影响因子:0.365; 五年影响因子:0.359 )
ISSN: 1680-5593
年卷期: 2012 年 11 卷 24 期
页码:
收录情况: SCI
摘要: Peroxisome Proliferator-Activated Receptor gamma (PPAR-gamma) was a key regulator of proliferation and preadipocyte differentiation in mammals. The objective of this study was to investigate the effect of the PPAR-gamma gene on slaughter traits of 170 individuals from Zhejiang White geese (ZW) and the ontogenetic expression pattern. PCR-SSCP technique was developed to analyze coding region of the PPAR-gamma gene polymorphism. The results showed that two genotypes (AA and AG) which were the products of two alleles (A and G) were observed. Association analysis indicated that this SNP was significantly associated with percentage of goose leg muscle (p<0.05) and the allele A was the predominant allele. Quantitative real-time PCR (qRT-PCR) assay measured the PPAR-gamma mRNA expression in ZW and Landes goose five tissues of different ages (P1, P14, P21, P28, P35, P42, P49, P56 and P63). The results showed that the PPAR-gamma mRNA had the highest expression level in goose abdominal fat and subcutaneous fat followed by lung and intestine. The PPAR-gamma mRNA levels exhibited a rise-decline change in fat tissues except for ZW subcutaneous fat. The values in fat tissues were higher than those of other tissues at the same goose breed (p<0.01). Besides, average values in. ZW fat and liver tissues were higher than those of Landes goose totally. These results suggested that the PPAR-gamma expressions were positively associated with goose fat development and had a breed-related tendency. The PPAR-gamma gene polymorphism could be used in Marker Assistant Selection (MAS) as a genetic marker for goose slaughter traits.
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