您好,欢迎访问上海市农业科学院 机构知识库!

Ontogenic Expression Pattern and Genetic Polymorphism of the Peroxisome Proliferator-Activated Receptor (PPAR-gamma) Gene in Goose Populations

文献类型: 外文期刊

作者: Wu, Hua-Li 1 ; Wang, Hui-Ying 1 ; He, Da-Qian 1 ; Liu, Yi 1 ; Gong, Shao-Ming 1 ; Kong, Fan-Li 2 ; Zhu, Qing 2 ;

作者机构: 1.Shanghai Acad Agr Sci, Inst Anim Husb & Vet Sci, Shanghai 201106, Peoples R China

2.Sichuan Agr Univ, Coll Anim Sci & Technol, Lab Anim Genet & Breeding, Yaan 625014, Sichuan, Peoples R China

关键词: Goose;PPAR-gamma gene;SNP;carcass traits;expression

期刊名称:JOURNAL OF ANIMAL AND VETERINARY ADVANCES ( 影响因子:0.365; 五年影响因子:0.359 )

ISSN: 1680-5593

年卷期: 2012 年 11 卷 24 期

页码:

收录情况: SCI

摘要: Peroxisome Proliferator-Activated Receptor gamma (PPAR-gamma) was a key regulator of proliferation and preadipocyte differentiation in mammals. The objective of this study was to investigate the effect of the PPAR-gamma gene on slaughter traits of 170 individuals from Zhejiang White geese (ZW) and the ontogenetic expression pattern. PCR-SSCP technique was developed to analyze coding region of the PPAR-gamma gene polymorphism. The results showed that two genotypes (AA and AG) which were the products of two alleles (A and G) were observed. Association analysis indicated that this SNP was significantly associated with percentage of goose leg muscle (p<0.05) and the allele A was the predominant allele. Quantitative real-time PCR (qRT-PCR) assay measured the PPAR-gamma mRNA expression in ZW and Landes goose five tissues of different ages (P1, P14, P21, P28, P35, P42, P49, P56 and P63). The results showed that the PPAR-gamma mRNA had the highest expression level in goose abdominal fat and subcutaneous fat followed by lung and intestine. The PPAR-gamma mRNA levels exhibited a rise-decline change in fat tissues except for ZW subcutaneous fat. The values in fat tissues were higher than those of other tissues at the same goose breed (p<0.01). Besides, average values in. ZW fat and liver tissues were higher than those of Landes goose totally. These results suggested that the PPAR-gamma expressions were positively associated with goose fat development and had a breed-related tendency. The PPAR-gamma gene polymorphism could be used in Marker Assistant Selection (MAS) as a genetic marker for goose slaughter traits.

  • 相关文献

[1]Molecular characterization, expression profile, and polymorphism of goose dopamine D1 receptor gene. Wang, Cui,Liu, Yi,Wang, Huiying,Wu, Huali,Gong, Shaoming,He, Daqian.

[2]Cloning, expression, purification, and characterization of an organophosphate-degrading enzyme in Escherichia coli. Jinbin Wang,Dachao Chen,Dongxiao Hu,Xuefeng Su,Xueming Tang. 2013

[3]CHEMICAL SYNTHESIS OF TOXIN DOMAIN OF CRYIA (c) GENE AND EXPRESSION IN ESCHERICHIA COLI. Rihe Peng,Aisheng Xiong,Xian Li,Huiqin Fan,Quanhong Yao. 2001

[4]Cloning and Characterization of Goose PPAR gamma Gene Comparison with Other Vertebrates. Wu, H. L.,Kong, F. L.,Qiu, M. H.,Zhou, B.,Luo, J.,Zhu, Q.,Wang, H. Y.,Liu, Y.,He, D. Q.. 2012

[5]绿壳蛋鸡与白来航蛋鸡microRNA变异及其靶基因预测分析. 管毓渝,廖荣荣,王振,赵静,陈振亮,张哲,肖倩,孙浩,王起山. 2016

[6]基于GBS技术的新杨绿壳纯系蛋鸡SNPs检测. 张彩云,王晓亮,涂盈盈,叶绍辉,姚俊峰,杨长锁. 2018

[7]斑玉蕈栽培菌株遗传变异与生长速率GWAS分析. 龚明,李燕,鲍大鹏,尚俊军,杨瑞恒,周陈力,万佳宁,吴莹莹. 2021

[8]基于限制性内切酶的简化基因组测序在家禽基因组研究中的应用现状. 张彩云,朱丽慧,姚俊峰,杨长锁. 2018

[9]糯玉米‘沪五彩花糯1号’品系特异性2b-RAD分子标记的开发及应用. 李全林,王义发,韩晴,袁政,沈雪芳. 2016

[10]基于SNPs标记的猪肉DNA溯源技术的研究. 张小波,吴潇,何慧,朱连龙,唐雪明. 2011

[11]基于SNP分型的香菇交配型AS-PCR鉴定. 张美彦,宋春艳,于海龙,谭琦,徐珍,王瑞娟,章炉军,尚晓冬. 2019

[12]浙东白鹅IGF1基因克隆、组织表达及与体重性状关联分析. 张雨婷,王翠,刘毅,杨云周,王惠影,李光全,王贤泽,龚绍明,何大乾. 2023

[13]利用PCR产物混池重测序检测梨褐皮关联SLAF标签多态性. 蒋爽,王晓庆,施春晖,李水根,骆军. 2024

[14]基于RFLP-PCR的猪肉溯源技术研究. 吴华莉,涂尾龙,曹建国,吴潇,常华,都启晶,谈永松. 2017

[15]香菇"沪香F2"自交群体产量等性状的GWAS分析. 杨沁閻,李巧珍,向泉桔,尚晓冬,章炉军,辜运富. 2024

[16]Genetic Diversity of the Melanocortin 4 Receptor (MC4R) Gene and its Association with Slaughter Traits in the Landes Goose. Huang, Yi,Wang, Yan,Liu, Yiping,Huang, Yi,He, Daqian. 2010

[17]Estimating number of transgene copies in transgenic rapeseed by real-time PCR assay with HMG I/Y as an endogenous reference gene. Weng, H,Pan, AH,Yang, LT,Zhang, CM,Liu, ZL,Zhang, DB.

[18]Isolated and characterization of a cDNA encoding ethylene-responsive element binding protein (EREBP)/AP2-type protein, RCBF2, in Oryza sativa L.. Liu, Jin-Ge,Zhang, Zhen,Qin, Qiu-Lin,Peng, Ri-He,Xiong, Ai-Sheng,Chen, Jian-Min,Xu, Fang,Zhu, Hong,Yao, Quan-Hong.

[19]High-level Expression of a Manganese Superoxide Dismutase (PoMn-SOD) from Pleurotus ostreatus in Pichia pastoris. Yin, Chaomin,Zhao, Wenxia,Ma, Aimin,Zheng, Liesheng,Chen, Liguo,Tan, Qi,Shang, Xiaodong.

[20]Cloning, expression, purification, and characterization of an organophosphate-degrading enzyme in Escherichia coli. Wang, Jinbin,Chen, Dachao,Hu, Dongxiao,Su, Xuefeng,Tang, Xueming. 2013

作者其他论文 更多>>