文献类型： 外文期刊

作者： Ping, Zhang Zhi ¹ ; Ming, Sheng ¹ ; Chui, Li ¹ ; Er, Sun Xin ¹ ; Hong, Yao Quan ² ; Ju, Wang Liang ¹ ;

作者机构： 1.Nanjing Agr Univ, Coll Horticulture, Nanjing 210095, Jiangsu, Peoples R China

2.Shanghai Acad Agr Sci, Agro Biotechnol Res Ctr, Shanghai 201106, Peoples R China

关键词： 5-aminolevulinic acid (ALA);Arabidopsis thaliana HemA1 promoter;Saccharomyces cerevisiae Hem1 gene;Solanum lycopersicum L.

期刊名称：XXVIII INTERNATIONAL HORTICULTURAL CONGRESS ON SCIENCE AND HORTICULTURE FOR PEOPLE (IHC2010): INTERNATIONAL SYMPOSIUM ON PLANT PHYSIOLOGY FROM CELL TO FRUIT PRODUCTION SYSTEM

ISSN： 0567-7572

年卷期： 2012 年 932 卷

页码：

收录情况： SCI

摘要： A gene encoding 5-aminolevulinic acid synthase (ALA-S) in Saccharomyces cerevisiae (Hem1) under the control of an Arabidopsis thaliana HemA1 promoter (HemA1 promoter) was introduced into the genome of tomato (Solanum lycopersicum). All transgenic tomato plants were able to transcribe the Hem1 gene and the capacity to synthesize ALA was increased. The transgenic plants had 18% higher levels of chlorophylls, as compared to wild-type plants, indicating that part of the additional ALA from yeast ALA-S was metabolized to chlorophylls. Determination of gas exchange suggested that the net photosynthetic rate (P-n) in the transgenic tomato was about 60% higher than that of wild type. The apparent quantum yield (AQY) in the transgenic plants was 0.0363, significantly higher than 0.0313 of wild type. The Chlorophyll-a fast fluorescence measurement and JIP-test at continuous intensities of excitation light (90-2100 mu mol m(-2) s(-1)) showed that the genetic transformation caused a significant reduction in V-j (the relative variable fluorescence at the J-step) but addition in V-i (the relative variable fluorescence at the I-step). The maximum photochemical efficiency (phi(Po)=F-v/F-m) did not vary with the light intensity, which demonstrated that phi(Po) was a stable index for leaf photosynthesis. psi(o) (the probability that a trapped exciton moves an electron into the electron transport chain beyond Q(A)(-)) and phi(Eo) (the quantum yield of electron transport) decreased with the light intensity increased, especially in wild type. Furthermore, the photosynthetic performance index on absorption basis (PIABS) or cross section basis (PICS) in the transgenic plants was also increased obviously in transgenic tobacco. Thus, the results obtained in this work approved that ALA improved photosynthetic capacity and the activity of PSII reaction center.