Studies on genetic relationship analysis and purity identification of hybrids of melon cultivars by SRAP markers
文献类型： 外文期刊
作者： Zhang, Y. P. 1 ; Yang, S. J. 1 ; Chen, Y. Y. 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Hort Res, Shanghai Key Lab Hort Protect Technol, Shanghai 201403, Peoples R China
关键词： SRAP;melon;genetic relationship analysis;hybrid seed purity
期刊名称：CUCURBITACEAE 2012: PROCEEDINGS OF THE XTH EUCARPIA MEETING ON GENETICS AND BREEDING OF CUCURBITACEAE
ISSN：
年卷期： 2012 年
页码：
收录情况： SCI
摘要： Genetic relationship analysis of twelve melon cultivars and the identification of hybrid seed purity of a melon cultivar were analyzed with SRAP technology. Our results show that 13 out of 40 SRAP primers were screened and 78 polymorphic bands were amplified, with a polymorphic band percentage of 50%. Clustering analysis showed that the value of genetic variation indexes of 12 melon cultivars based on the SRAP data varied from 0.263 to 0.921, and they could be classified into 2 groups and 5subgroups by UPGMA phylogenetic analysis. Primers Me15-Em7 and Me3-Em16 were selected for SRAP identification of 100 single seeds of 'Honglvzaocui', and the purity was 77%. This study would provide information for genetic relationship analysis and identification of hybrid seed purity of melon cultivars.
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