文献类型： 外文期刊
作者： Yi, J. 1 ; Liu, Ch. 1 ;
作者机构： 1.Shanghai Acad Agr Sci, Inst Anim Husb Vet Sci, Shanghai 201106, Peoples R China
关键词： Foot-and-mouth disease virus;virus-cell binding;RNase A;RT-PCR;VP1 protein
期刊名称：ACTA VIROLOGICA （ 影响因子：1.162； 五年影响因子：1.255 ）
ISSN： 0001-723X
年卷期： 2010 年 54 卷 4 期
页码：
收录情况： SCI
摘要： In this study, we examined the integrity of Foot-and-mouth disease virus (FMDV) particles during their binding to the surface of BHK-21 cells under physiological condition. For monitoring of the virus integrity we used blocking of the endocytosis with dynasore and cytochalasin D followed by RT-PCR for viral protein VP1 and the resistance of FMDV to the treatment of RNase A. Our results showed that integrin binding to VP1 did not cause a substantial conformational change in the viral capsid. Furthermore, treatment with RNase A showed no effect on the infectivity of intact as well as cell-bound virions. Our findings confirmed that FMDV entered the host cells in the form of intact virions.
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