文献类型： 外文期刊

作者： Miao Jin-feng ¹ ; Zhang Yuan-shu ¹ ; Huang Guo-qing ¹ ; Ma Hai-tian ¹ ; Zou Si-xiang ¹ ; Zhu Yu-min ² ;

作者机构： 1.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Peoples R China

2.Shanghai Acad Agr Sci, Inst Anim Sci & Vet Med, Shanghai 201106, Peoples R China

关键词： lipopolysaccharide;rat mastitis;Th1/Th2;BCG-PSN

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2009 年 8 卷 8 期

页码：

收录情况： SCI

摘要： The aim of this study was to evaluate, in rats, the changes in the T helper type 1 (Th1)/Th2 radio in mammary glands after an intramammary infusion of lipopolysaccharide (LPS) and to characterize the moderating effects of the polysaccharide nucleic acid of Bacillus Calmetre Guerin (BCG-PSN) on the mammary gland. In the control group, the levels of IL-2 and INF-gamma mRNA expression increased, whereas IL-4 mRNA expression decreased after LPS challenge. As a consequence, the INF-gamma/IL-4 mRNA ratio was significantly higher at 3, 6, and 9 h post-infusion (PI) compared to the control value (0 h; P<0.01). BCG-PSN increased mRNA expression of both INF-gamma and IL-4 before infusion of LPS. LPS challenge significantly the reduced Th1/Th2 cytokine ratio due to Th1 cytokine IFN-gamma suppression and Th2 cytokine IL-4 upregulation compared with the control group. A significant reduction of N-acetyl-beta-D-glucosaminidase (NAGase) was observed at 24 h PI in the BCG-PSN treatment group compared to the control group (P<0.05). Thus, it was demonstrated that level of BCG-PSN might change the Th1/Th2 ratio mainly by enhancing the Th2 immune response. This is the first report of a Th1/Th2 change induced by coliform mastitis and characterization of the effect of BCG-PSN on mammary gland inflammation. This study makes a better understanding of the mechanisms of coliform mastitis and provides a putative novel strategy for the prevention and/or treatment of mastitis.