文献类型： 外文期刊

作者： Wang Xinhua ¹ ; Chen Huoying ¹ ; Zhu Yuying ² ; Hou Ruixian ² ;

作者机构： 1.Shanghai Jiao Tong Univ, Sch Agr & Biol, Shanghai 200240, Peoples R China

2.Shanghai Acad Agr Sci, Protected Hort Res Inst, Shanghai 201106, Peoples R China

关键词： Pak-choi;TuMV resistance;AFLP;BSA

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2009 年 8 卷 11 期

页码：

收录情况： SCI

摘要： Pak-choi is one of the most important vegetable crops in China. Turnip mosaic virus (TuMV) is one of its main pathogen. Screening the molecular marker linked to the TuMV resistance gene is an efficient method to improve pak-choi breeding. In this paper, a dominant gene, TuRBCH01, has been mapped. 180 F(2) individuals were inoculated with TuMV-C5 and tested by direct ELISA. The 3: 1 ratio of F(2) hybrids segregation proved a single dominant allele for TuMV resistance. Amplified fragment length polymorphism (AFLP) technique and bulked segregant analysis (BSA) method were used to study the F(2) population. An AFLP marker (EccMctt3) linked to TuMV resistance gene with 7.8cM map distance was identified.