您好，欢迎访问上海市农业科学院 机构知识库！
上海市农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Expression analysis of a novel pyridoxal kinase messenger RNA splice variant, PKL, in oil rape suffering abiotic stress and phytohormones

文献类型： 外文期刊

作者： Yu, Shunwu 1 ; Luo, Lijun 1 ;

作者机构： 1.Shanghai Agrobiol Gene Ctr, Shanghai Acad Agr Sci, Germsperm Resources Div Shanghai, Natl Key Lab Crop Genet Improvement, Shanghai 201106, Peoples R China

关键词： pyridoxal kinase;abiotic stress;phytohormone;Brassica napus;splice

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.848； 五年影响因子：3.67 ）

ISSN： 1672-9145

年卷期： 2008 年 40 卷 12 期

页码：

收录情况： SCI

摘要： Pyridoxal kinase is key enzyme for the biosynthesis of pyridoxa 15'-phosphate, the biologically active form of vitamin B6, in the salvage pathway. A pyridoxal kinase gene, BnPKL (GenBank accession No. DQ 463962), was isolated from oilseed rape (Brassica napus L.) following water stress through rapid amplification of complementary DNA (cDNA) ends. The results showed that the gene had two splice variants: PKL and PKL2. PKL, the long cDNA, encodes a 334 amino acid protein with a complete ATP-binding site, pyridoxal kinase-binding site and dimer interface site of a pyridoxal kinase, while PKL2, the short cDNA, lacked a partial domain. Southern blot showed that there were two copies in Brassica napus. The expression of BnPKL cDNA could rescue the mutant phenotype of Escherichia coli defective in pyridoxal kinase. Real-time reverse transcription-polymerase chain reaction revealed that the relative abundance of two transcripts are modulated by development and environmental stresses. Abscisic acid and NaCl were inclined to decrease PKL expression, but H(2)O(2) and cold temperatures induced the PKL expression. In addition, the PKL expression could be transiently induced by jasmonate acid at an early stage, abscisic acid, salicylic acid and jasmonate acid enhanced the PKL expression in roots. Our results de monstrated that BnPKL was a pyridoxal kinase involved in responses to biotic and abiotic stresses.

  • 相关文献

[1]Isolation and characterization of BnMKK1 responsive to multiple stresses and affecting plant architecture in tobacco. Yu, Shunwu,Chen, Chen,Li, Jiajia,Ye, Shuifeng,Liu, Guolan,Mei, Xiaohan,Luo, Lijun,Zhang, Lida,Tang, Kexuan.

[2]A liquid chromatography tandem mass spectrometry method for simultaneous determination of acid/alkaline phytohormones in grapes. Liu, Hong,Han, Zheng,Liu, Gang,Rao, Qinxiong,Bai, Bing,Zhao, Zhihui,Wu, Aibo.

[3]Estimating number of transgene copies in transgenic rapeseed by real-time PCR assay with HMG I/Y as an endogenous reference gene. Weng, H,Pan, AH,Yang, LT,Zhang, CM,Liu, ZL,Zhang, DB.

[4]Codon-modifications and an endoplasmic reticulum-targeting sequence additively enhance expression of an Aspergillus phytase gene in transgenic canola. Peng, RH,Yao, QH,Xiong, AS,Cheng, ZM,Li, Y. 2006

[5]Isolation and characterization of an oilseed rape MAP kinase BnMPK3 involved in diverse environmental stresses. Yu, SW,Zhang, LD,Zuo, KJ,Tang, DQ,Tang, KX.

[6]Expression and Function of a Modified AP2/ERF Transcription Factor from Brassica napus Enhances Cold Tolerance in Transgenic Arabidopsis. Xiong, Ai-Sheng,Wang, Feng,Jiang, Hai-Hua,Peng, Ri-He,Jin, Xiao-Fen,Zhu, Bo,Yao, Quan-Hong,Zhuang, Jing,Zhang, Jian.

[7]Salicylic Acid and Abiotic Stress Responses in Rice. Pal, M.,Kovacs, V.,Szalai, G.,Soos, V.,Janda, T.,Ma, X.,Liu, H.,Mei, H.. 2014

[8]Expression of a rice DREB1 gene, OsDREB1D, enhances cold and high-salt tolerance in transgenic Arabidopsis. Zhang, Yang,Chen, Chen,Jin, Xiao-Fen,Xiong, Ai-Sheng,Peng, Ri-He,Yao, Quan-Hong,Zhang, Yang,Chen, Chen,Hong, Yi-Huan,Chen, Jian-Min.

[9]Isolation, Phylogeny and Expression Patterns of AP2-Like Genes in Apple (Malus x domestica Borkh). Zhuang, Jing,Yao, Quan-Hong,Xiong, Ai-Sheng,Zhuang, Jing,Zhang, Jian.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：上海市农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台