Efficient transformation of Penicillium chrysogenum mediated by Agrobacterium tumefaciens LBA4404 for cloning of Vitreoscilla hemoglobin gene
文献类型： 外文期刊
作者： Sun, CB 1 ; Kong, QL 2 ; Xu, WS 2 ;
作者机构： 1.Shanghai Inst Pharmaceut Ind, Lab Genet Engn, Dept Biopharmaceut, Shanghai 200040, Peoples R China
2.Shanghai Inst Pharmaceut Ind, Lab Genet Engn, Dept Biopharmaceut, Shanghai 200040, Peoples R China; Shanghai Acad Agr Sci, Dept Proc & Store Agroprod, Shanghai 201106, Peoples R China
关键词： Agrobacterium tumefaciens;Penicillium chrysogenum;Ti plasmid;transformation
期刊名称：ELECTRONIC JOURNAL OF BIOTECHNOLOGY （ 影响因子：2.8； 五年影响因子：3.379 ）
ISSN： 0717-3458
年卷期： 2002 年 5 卷 1 期
页码：
收录情况： SCI
摘要： The vgb and bleomycin resistance genes could be efficiently transferred into the filamentous fungus Penicillium chrysogenum under help of T-DNA of Agrobacterium tumefaciens LBA4404 and the transferred genes were integrated at a chromosomal locus of P. chrysogenum. Transformation using A. tumefaciens LBA4404 could be enhanced in the presence of acetosyringone (AS) when being carried out in the conidiaand mycelium. The efficiencies of transformation could be improved up to 10 folds compared with conventional techniques for transformation of P. chrysogenum protoplasts.
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