文献类型： 外文期刊

作者： Li, GJ ¹ ; Li, YJ ² ; Yan, WY ² ; Xu, QX ² ; Wu, YQ ² ; Xie, Y ² ; You, YJ ² ; Zheng, ZX ² ;

作者机构： 1.Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200433, Peoples R China

2.Fudan Univ, Sch Life Sci, State Key Lab Genet Engn, Shanghai 200433, Peoples R China; Shanghai Acad Agr Sci, Inst Vet Sci, Shanghai 201106, Peoples R China; Hong Kong Univ Sci & Technol, Dept Biol, Hong Kong, Hong Kong, Peoples R China

关键词： CpG DNA;DNA vaccine;foot-and-mouth disease virus;immnostimulatory sequence

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN： 1001-6538

年卷期： 2001 年 46 卷 16 期

页码：

收录情况： SCI

摘要： CpG DNA is DNA sequence that has immune stimulatory effects. Several tines of Investigation over the past few years indicate that CpG DNA plays an important role in the induction of immune responses to DNA vaccines. In this study, CpG DNA-containing synthetic oligodeoxynucleotide (CpG-ODN) was cloned into the eukaryotic expression plasmid encoding a fusion protein containing beta- galactosidase from E. coli and immunogenic epitopes of foot-and-mouth disease virus (FMDV) type O, and the immune responses induced by the plasmid were assayed. The results showed that guinea pigs immunized with the recombinant plasmid containing CpG-ODN generated a higher level of FMDV-neutralizing antibody and a stronger T cell proliferative response and protection against viral challenge than those receiving the plasmid containing no CpG-ODN. Our study demonstrated that it is an effective route to enhance the efficacy of DNA vaccines by inserting exogenous CpG DNA into the plasmids, and the DNA vaccine developed here Is a promising candidate to prevent FMDV infection.