文献类型： 外文期刊

作者： Yang, ZP ¹ ; Yang, XY ² ; Huang, DC ² ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Plant Protect, Shanghai 201106, Peoples R China

2.Shanghai Acad Agr Sci, Inst Plant Protect, Shanghai 201106, Peoples R China; Shanghai Acad Agr Sci, AgroBiotechnol Res Ctr, Shanghai 201106, Peoples R China

关键词： bread wheat;Triticum aestivum;tolerance to deoxynivalenol;somaclonal variant;in vitro selection

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 1998 年 101 卷 2 期

页码：

收录情况： SCI

摘要： This study was conducted to develop an efficient in vitro selection system for scab resistance by using in vitro screening for tolerance to deoxynivalenol (DON). Immature embryos of two wheat varieties, a scab-resistant variety Sumai 3 and a susceptible variety Mianyang 11, and their reciprocal F-1 hybrids were cultured on MS medium supplemented with 2,4-D 2 mg/l and 0.6 x 10(-4) M DON for callus induction. The responses of callus induction and plant regeneration to 0.6 x 10(-4) M DON differed significantly between resistant and susceptible varieties, according to observed scab resistance levels at the plant level in the field. The percentage of callus formation of resistant variety Sumai 3 on induction medium containing DON was higher than that of susceptible variety Mianyang 11. Regeneration of DON-tolerant calli on DON-containing differentiation medium differed significantly between Sumai 3 and Mianyang 11. Averaged across the DON-tolerant calli of two varieties and their reciprocals, regeneration of DON-tolerant calli was decreased 3-fold on DON-containing medium. By an inoculation test with conidiospores of Fusarium graminearum Schw, we obtained several resistant lines from progenies of regenerated plants from DON-tolerant calli. These somaclonal lines had lower disease scoring (reaction index, infected spikelets and disease incidence), shorter plants and better yield components than Sumai 3, a famous Chinese resistant variety.