Genetic homogeneity of cultivated strains of shiitake (Lentinula edodes) used in China as revealed by the polymerase chain reaction
文献类型： 外文期刊
作者： Chiu, SW 1 ; Ma, AM 2 ; Lin, FC 2 ; Moore, D 2 ;
作者机构： 1.SHANGHAI ACAD AGR SCI,EDIBLE FUNGI INST,SHANGHAI,PEOPLES R CHINA
2.SHANGHAI ACAD AGR SCI,EDIBLE FUNGI INST,SHANGHAI,PEOPLES R CHINA; HUAZHONG AGR UNIV,INST APPL MYCOL,WUHAN,PEOPLES R CHINA; UNIV MANCHESTER,SCH BIOL SCI,MANCHESTER M13 9PT,LANCS,ENGLAND
期刊名称：MYCOLOGICAL RESEARCH （ 影响因子：2.809； 五年影响因子：2.923 ）
ISSN： 0953-7562
年卷期： 1996 年 100 卷
页码：
收录情况： SCI
摘要： Nineteen strains of shiitake (Lentinula edodes) which are used for spawn production for farms throughout mainland China were characterized by three arbitrarily-primed polymerase chain reaction (AP-PCR) profiles, seven random amplified polymorphic DNA marker (RAPD) profiles and Eve restriction patterns (restriction fragment length polymorphism patterns of the PCR-amplified ribosomal DNAs; rDNA-RFLPs). For AP-PCR, 4 - 14 DNA bands were amplified for a particular strain while I - 9 DNA bands were amplified using RAPD. Among them only three of the strains tested showed different amplification profiles with most of the primers used. The others showed small differences in three or fewer DNA amplification profiles. All strains showed identical rDNA-RFLPs. The study indicates that cultivated strains of shiitake in China are genetically very homogeneous, very like cultivated strains of Agaricus bisporus and Volvariella volvacea. However, our collection of L. edodes covers an enormous geographical area (approx. 1700 km N to S, 700 km E to W) and our results demonstrate that the shiitake industry in China depends on an extremely small gene pool. In comparison, three wild strains collected from the same ecosystem in Fujian Province (SE China) showed more variable amplification profiles. This emphasises the importance of conservation of wild shiitake strains as a reserve of biodiversity for this important industry.
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