Lytic Bacteriophage PZL-Ah152 as Biocontrol Measures Against Lethal Aeromonas hydrophila Without Distorting Gut Microbiota
文献类型： 外文期刊
作者： Feng, Chao 1 ; Jia, Kaixiang 1 ; Chi, Teng 1 ; Chen, Shuaimin 2 ; Yu, Huabo 1 ; Zhang, Liang 1 ; Haidar Abbas Raza, Sayed 3 ; Alshammari, Ahmed Mohajja 4 ; Liang, Shuang 1 ; Zhu, Zishan 1 ; Li, Tingxuan 1 ; Qi, Yanling 1 ; Shan, Xiaofeng 1 ; Qian, Aidong 1 ; Zhang, Dongxing 1 ; Zhang, Lei 1 ; Sun, Wuwen 1 ;
作者机构： 1.Jilin Agr Univ, Coll Anim Sci & Technol, Jilin, Peoples R China
2.Jilin Acad Agr Sci, Inst Agr Resources & Environm, Changchun, Peoples R China
3.Northwest A&F Univ, Coll Anim Sci & Technol, Xianyang, Peoples R China
4.Univ Hail, Coll Sci, Dept Biol, Hail, Saudi Arabia
关键词： Aeromonas hydrophila; phage therapy; phage genome; phage safety; gut microbiota
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：6.064； 五年影响因子：6.843 ）
ISSN：
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： Phage therapy is an alternative approach to overcome the problem of multidrug resistance in bacteria. In this study, a bacteriophage named PZL-Ah152, which infects Aeromonas hydrophila, was isolated from sewage, and its biological characteristics and genome were studied. The genome contained 54 putative coding sequences and lacked known putative virulence factors, so it could be applied to phage therapy. Therefore, we performed a study to (i) investigate the efficacy of PZL-Ah152 in reducing the abundance of pathogenic A. hydrophila strain 152 in experimentally infected crucian carps, (ii) evaluate the safety of 12 consecutive days of intraperitoneal phage injection in crucian carps, and (iii) determine how bacteriophages impact the normal gut microbiota. The in vivo and in vitro results indicated that the phage could effectively eliminate A. hydrophila. Administering PZL-Ah152 (2 x 10(9) PFU) could effectively protect the fish (2 x 10(8) CFU/carp). Furthermore, a 12-day consecutive injection of PZL-Ah152 did not cause significant adverse effects in the main organs of the treated animals. We also found that members of the genus Aeromonas could enter and colonize the gut. The phage PZL-Ah152 reduced the number of colonies of the genus Aeromonas. However, no significant changes were observed in alpha-diversity and beta-diversity parameters, which suggested that the consumed phage had little effect on the gut microbiota. All the results illustrated that PZL-Ah152 could be a new therapeutic method for infections caused by A. hydrophila.
- 相关文献
作者其他论文 更多>>
-
Combining time-variable controlled release urea formulations to improve spring maize yield and reduce nitrogen losses in northeastern China
作者：Hou, Yunpeng;Kong, Lili;Zhang, Lei;Wang, Lichun;Xu, Xinpeng;Zhang, Yitao
关键词：Controlled -release urea; Nitrogen loss; Nitrogen use efficiency; Soil inorganic nitrogen; Northeast China
-
Intradermal vaccination with Porcilis® Begonia can clinically protect against fatal PRV challenge with the highly virulent ZJ01 field strain
作者：Zhang, Lei;Liu, Fei;Zhang, Lei;Liu, Bei-Bei;Li, Wen-Liang;Hao, Fei;Wang, Jia;Gan, Yuan;Yuan, Ting;Feng, Zhi-Xin;Shao, Guo-Qing;van den Born, Erwin;Segers, Ruud P. A. M.;Raes, Maurice;Di, Di;Zhang, Lei;Liu, Bei-Bei;Li, Wen-Liang;Hao, Fei;Wang, Jia;Gan, Yuan;Yuan, Ting;Feng, Zhi-Xin;Shao, Guo-Qing
关键词：Pseudorabies virus; Intradermal; Vaccination; Pigs; Challenge
-
Stable isotope probing reveals compositional and functional shifts in active denitrifying communities along the soil profile in an intensive agricultural area
作者：Chen, Shuaimin;Liu, Binbin;Chen, Shuaimin;Kuramae, Eiko E.;Chen, Shuaimin;Kuramae, Eiko E.;Kuramae, Eiko E.;Jia, Zhongjun
关键词：Nitrate leaching; Denitrification; Stable isotope probing; Vadose zone; Active denitrifiers
-
Film-mulched drip irrigation achieves high maize yield and low N losses in semi-arid areas of northeastern China
作者：Hou, Yunpeng;Kong, Lili;Zhang, Lei;Wang, Lichun;Xu, Xinpeng;Zhang, Yitao
关键词：Water and nitrogen management; Film -mulched drip irrigation; Nitrogen loss; Nitrogen use efficiency
-
Recombination Variation Shapes Phylogeny and Introgression in Wild Diploid Strawberries
作者：Feng, Chao;Kang, Ming;Feng, Chao;Kang, Ming;Wang, Jing;Liston, Aaron
关键词：Fragaria; introgression; mating system; phylogenomics; recombination rate; wild diploid strawberry
-
Mucosal and cellular immune responses elicited by nasal and intramuscular inoculation with ASFV candidate immunogens
作者：Xu, Lulu;Liu, Yongjie;Feng, Zhixin;Xie, Xing;Xu, Lulu;Hao, Fei;Chen, Rong;Gan, Yuan;Zhang, Lei;Yu, Yanfei;Bai, Yun;Xiong, Qiyan;Shao, Guoqing;Wu, Yuzi;Feng, Zhixin;Xie, Xing;Xu, Lulu;Hao, Fei;Chen, Rong;Gan, Yuan;Zhang, Lei;Yu, Yanfei;Bai, Yun;Xiong, Qiyan;Shao, Guoqing;Wu, Yuzi;Feng, Zhixin;Xie, Xing;Hao, Fei;Feng, Zhixin;Xie, Xing;Jeong, Dae Gwin;Yeom, Minjoo;Lim, Jong-Woo;Song, Daesub;Yeom, Minjoo;Lim, Jong-Woo;Song, Daesub;Zeng, Zhiyong
关键词：African swine fever virus (ASFV); ASFV-convalescent pig serum; mass spectrometry identification; recombinant protein expression; immunogenicity assessment
-
Rapid and ultra-sensitive detection of African swine fever virus antibody on site using QDM based-ASFV immunosensor (QAIS)
作者：Li, Jiahao;Zhang, Yue;Shan, Yanke;Liu, Fei;Li, Jiahao;Zhang, Yue;Shan, Yanke;Liu, Fei;Bai, Yun;Xie, Qingyun;Zhang, Lei;Hua, Lizhong;Xiong, Qiyan;Shao, Guoqing;Feng, Zhixin;Li, Fang;Bu, Zhigao;Zhao, Dongming
关键词：African swine fever virus (ASFV) antibodies; Dual quantum dot microsphere (QDM) probes; Pre-incubation; QDM based-ASFV immunosensor (QAIS); Clinical samples; On-site testing
