Characterization of a Novel C-type Lectin against OsHV-1 infection in Scapharca broughtonii
文献类型： 外文期刊
作者： Wang, D. 1 ; Huang, B. 2 ; Yu, J. 2 ; Bai, C. 2 ; Li, C. 2 ; Wang, C. 2 ; Xin, L. 2 ; Zhou, H. 1 ;
作者机构： 1.Hainan Univ, Haikou 570228, Hainan, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China
3.Chinese Acad Fishery Sci, Qingdao Key Lab Mariculture Epidemiol & Biosecur, Key Lab Maricultural Organism Dis Control, Yellow Sea Fisheries Res Inst,Minist Agr, Qingdao 266071, Peoples R China
4.Dalian Ocean Univ, Dalian 116023, Peoples R China
关键词： Scapharca broughtonii; C-type lectin; PAMP binding; OsHV-1 infection
期刊名称：ISJ-INVERTEBRATE SURVIVAL JOURNAL （ 影响因子：0.917； 五年影响因子：1.263 ）
ISSN： 1824-307X
年卷期： 2022 年 19 卷
页码：
收录情况： SCI
摘要： As important pattern recognition receptors (PRRs), most C-type lectins (CTLs) are a class of Ca2+-dependent carbohydrate-binding proteins that are found to be involved in non-self-recognition and antiviral process. In this study, a new CTL, named SbCTL, was identified from ark clams, Scapharca broughtonii. The amino acid sequence of SbCTL consisted of a predicted CRD (carbohydrate recognition domain) structural domain (including 102 amino acid residues). The amino acid sequence of SbCTL shared 28 % - 39 % similarity with other CTLs. There were two potential Ca2+ binding sites in SbCTL. The expression of SbCTL mRNA was detected in all selected tissues, with the highest expression in the gills. Expression of SbCTL mRNA increased significantly (p < 0.05) and total vibrio content increased significantly (p < 0.05) in ark clam infected with OsHV-1 at 72 h post-infection. The binding activities of recombinant SbCTL (rSbCTL) to various PAMPs with or without Ca2+ were analyzed by ELISA, rSbCTL showed especially high binding activity to LPS in a Ca2+-independent manner. rSbCTL also functioned on sheltering ark clams from OsHV-1 infection in vivo, there were less mortality occurred in the rSbCTL treated group than the control. In all, it suggests that SbCTL, could served a critical role in the immune response against intruders in ark clams.
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